본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.20 no.6, 2010년, pp.1006 - 1010   SCIE 피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Effect of gcl, glcB, and aceA Disruption on Glyoxylate Conversion by Pseudomonas putida JM37

Li, Xuan Zhong    (School of Biotechnology and Biomolecular Sciences, The University of New South Wales   ); Klebensberger, Janosch    (School of Biotechnology and Biomolecular Sciences, The University of New South Wales   ); Rosche, Bettina    (School of Biotechnology and Biomolecular Sciences, The University of New South Wales  );
  • 초록

    Pseudomonas putida JM37 metabolized glyoxylate at a specific rate of 55 g/g dry biomass/day. In order to investigate their role, three genes encoding enzymes that are potentially involved in the conversion of glyoxylate were disrupted; namely, tartronate semialdehyde synthase (gcl), malate synthase (glcB), and isocitrate lyase (aceA). Strains with transposon insertion in either of these genes were isolated from a 50,000 clone library employing a PCR-guided enrichment strategy. In addition, all three double mutants were constructed via targeted insertion of a knock-out plasmid. Neither mutation of gcl, glcB, and aceA nor any of the respective double mutations influenced glyoxylic acid conversion, indicating that P. putida JM37 may possess other enzymes and pathways for glyoxylate metabolism.


  • 주제어

    Glyoxylic acid .   biotransformation .   Pseudomonas putida .   tartronate semialdehyde synthase .   malate synthase .   isocitrate lyase.  

  • 참고문헌 (25)

    1. Bailey, E. and R. P. Hullin. 1966. The metabolism of glyoxylate by cell-free extracts of Pseudomonas sp. Biochem. J. 101: 755-763. 
    2. Blackmore, M. A. and J. R. Quayle. 1970. Microbial growth on oxalate by a route not involving glyoxylate carboligase. Biochem. J. 118: 53-59. 
    3. dos Santos, V., S. Heim, E. R. B. Moore, M. Stratz, and K. N. Timmis. 2004. Insights into the genomic basis of niche specificity of Pseudomonas putida KT2440. Environ. Microbiol. 6: 1264-1286. 
    4. Gavagan, J. E., S. K. Fager, J. E. Seip, M. S. Payne, D. L. Anton, and R. Dicosimo. 1995. Glyoxylic-acid production using microbial transformant catalysts. J. Org. Chem. 60: 3957-3963. 
    5. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580. 
    6. Isobe, K. 1995. Oxidation of ethylene-glycol and glycolic acid by glycerol oxidase. Biosci. Biotechnol. Biochem. 59: 576-581. 
    7. Isobe, K. and H. Nishise. 1999. A method for glyoxylic acid production using cells of Alcaligenes sp. GOX373. J. Biotechnol. 75: 265-271. 
    8. Kanehisa, M. and S. Goto. 2000. KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 28: 27-30. 
    9. Kessler, B., V. Lorenzo, and K. N. Timmis. 1992. A general system to integrate lacZ fusions into the chromosomes of Gramnegative eubacteria: Regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy. Mol. Gen. Genet. 233: 293-301. 
    10. Klebensberger, J., K. Lautenschlager, D. Bressler, J. Wingender, and B. Philipp. 2007. Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy. Environ. Microbiol. 9: 2247-2259. 
    11. Kornberg, H. L. and A. M. Gotto. 1961. Metabolism of C2 compounds in micro-organisms. 6. Synthesis of cell constituents from glycollate by Pseudomonas sp. Biochem. J. 78: 69-82. 
    12. Kornberg, H. L. and J. G. Morris. 1965. The utilization of glycollate by Micrococcus denitrificans: The beta-hydroxyaspartate pathway. Biochem. J. 95: 577-586. 
    13. Kornberg, H. L. and J. R. Sadler. 1961. Metabolism of C2-compounds in micro-organisms. 8. A dicarboxylic acid cycle as a route for oxidation of glycollate by Escherichia coli. Biochem. J. 81: 503-513. 
    14. Lampe, D. J., B. J. Akerley, E. J. Rubin, J. J. Mekalanos, and H. M. Robertson. 1999. Hyperactive transposase mutants of the Himar1 mariner transposon. Proc. Natl. Acad. Sci. U.S.A. 96: 11428-11433. 
    15. Li, X. Z., B. Hauer, and B. Rosche. 2007. Single-species microbial biofilm screening for industrial applications. Appl. Microbiol. Biotechnol. 76: 1255-1262. 
    16. Merrett, M. J. and J. M. Lord. 1973. Glycollate formation and metabolism by algae. New Phytol. 72: 751-767. 
    17. Nelson, K. E., C. Weinel, I. T. Paulsen, R. J. Dodson, H. Hilbert, V. dos Santos, et al. 2002. Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ. Microbiol. 4: 799-808. 
    18. Pfennig, N. and K. D. Lippert. 1966. Uber das vitamin B12-bedurfnis phototropher schwefelbakterien. Arch. Mikrobiol. 55: 245-256. 
    19. Schomburg, I., A. Chang, C. Ebeling, M. Gremse, C. Heldt, G. Huhn, and D. Schomburg. 2004. BRENDA, the enzyme database: Updates and major new developments. Nucleic Acids Res. 32: D431-D433. 
    20. Seip, J. E., S. K. Fager, J. E. Gavagan, L. W. Gosser, D. L. Anton, and R. Dicosimo. 1993. Biocatalytic production of glyoxylic-acid. J. Org. Chem. 58: 2253-2259. 
    21. Simon, R., M. Oconnell, M. Labes, and A. Puhler. 1986. Plasmid vectors for the genetic-analysis and manipulation of Rhizobia and other Gram-negative bacteria. Methods Enzymol. 118: 640-659. 
    22. Windgassen, M., A. Urban, and K. E. Jaeger. 2000. Rapid gene inactivation in Pseudomonas aeruginosa. FEMS Microbiol. Lett. 193: 201-205. 
    23. Yadav, G. D. and V. R. Gupta. 2000. Synthesis of glyoxalic acid from glyoxal. Process Biochem. 36: 73-78. 
    24. Kornberg, H. L. and A. M. Gotto. 1959. Formation of malate from glycollate by Pseudomonas ovalis Chester. Nature 183: 1791-1793. 
    25. Sakai, S., K. Inokuma, Y. Nakashimada, and N. Nishio. 2008. Degradation of glyoxylate and glycolate with ATP synthesis by a thermophilic anaerobic bacterium, Moorella sp. strain HUC22-1. Appl. Environ. Microbiol. 74: 1447-1452. 
  • 이 논문을 인용한 문헌 (1)

    1. 2013. "" Journal of microbiology and biotechnology, 23(2): 195~204     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기