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Journal of microbiology and biotechnology v.20 no.6, 2010년, pp.1011 - 1017   SCIE 피인용횟수: 1
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New Dioscin-Glycosidase Hydrolyzing Multi-Glycosides of Dioscin from Absidia Strain

Fu, Yao Yao    (College of Science, Yanbian University   ); Yu, Hong Shan    (College of Bio & Food Technology, Dalian Polytechnic University   ); Tang, Si Hui    (College of Bio & Food Technology, Dalian Polytechnic University   ); Hu, Xiang Chun    (College of Bio & Food Technology, Dalian Polytechnic University   ); Wang, Yuan Hao    (College of Bio & Food Technology, Dalian Polytechnic University   ); Liu, Bing    (College of Bio & Food Technology, Dalian Polytechnic University   ); Yu, Chen Xu    (Department of Agricultural and Biosystems Engineering, Iowa State University   ); Jin, Feng Xie    (College of Bio & Food Technology, Dalian Polytechnic University  );
  • 초록

    A novel dioscin-glycosidase that specifically hydrolyzes multi-glycosides, such as 3-O- ${\alpha}$ -L-( $1{\to}4$ )-rhamnoside, 3-O- ${\alpha}$ -L-( $1{\to}2$ )-rhamnoside, 3-O- ${\alpha}$ -L-( $1{\to}4$ )-arabinoside, and ${\beta}$ -D-glucoside, on diosgenin was isolated from the Absidia sp.d38 strain, purified, and characterized. The molecular mass of the new dioscin-glycosidase is about 55 kDa based on SDS-PAGE. The dioscin-glycosidase gradually hydrolyzes either 3-O- ${\alpha}$ -L-( $1{\to}4$ )-Rha or 3-O- ${\alpha}$ -L-( $1{\to}2$ )-Rha from dioscin into 3-O- ${\alpha}$ -L-Rha- ${\beta}$ -D-Glc-diosgenin, further rapidly hydrolyzes the other ${\alpha}$ -L-Rha from 3-O- ${\alpha}$ -L-Rha- ${\beta}$ -D-Glc-diosgenin into the main intermediate products of 3-O- ${\beta}$ -D-Glc-diosgenin, and subsequently hydrolyzes these intermediate products into aglycone as the final product. The enzyme also gradually hydrolyzes 3-O- ${\alpha}$ -L-( $1{\to}4$ )-arabinoside, 3-O- ${\alpha}$ -L-( $1{\to}2$ )-rhamnoside, and ${\beta}$ -D-glucoside from [3-O- ${\alpha}$ -L-( $1{\to}4$ )-Ara, 3-O- ${\alpha}$ -L-( $1{\to}4$ )-Rha]- ${\beta}$ -D-Glc-diosgenin into diosgenin as the final product, exhibiting significant differences from previously reported glycosidases. The optimal temperature and pH for the new dioscin-glycosidase is $40^{\circ}C$ and 5.0, respectively. Whereas the activity of the new dioscin-glycosidase was not affected by $Na^+$ , $K^+$ , and $Mg^{2+}$ ions, it was significantly inhibited by $Cu^{2+}$ and $Hg^{2+}$ ions, and slightly affected by $Ca^{2+}$ ions.


  • 주제어

    Dioscin .   dioscin-glycosidase .   hydrolyzing multiglycosides.  

  • 참고문헌 (19)

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  • 이 논문을 인용한 문헌 (1)

    1. 2011. "" Journal of microbiology and biotechnology, 21(10): 1057~1063     

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  • Jin, Feng Xie (3)

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