Monitoring of Possible Horizontal Gene Transfer from Transgenic Potatoes to Soil Microorganisms in the Potato Fields and the Emergence of Variants in Phytophthora infestans
To examine the possibility of horizontal gene transfer between transgenic potatoes and microorganisms in potato fields, the gene flow from transgenic potatoes containing the nucleoside diphosphate kinase 2 (NDPK2) gene to microorganisms in soils was investigated. The soil samples collected from the potato fields from March to October 2007 were examined by PCR, Southern hybridization, and AFLP fingerprinting. The NDPK2 gene from soil genomic DNAs was not detected by both PCR and Southern hybridization, indicating that gene transfer did not occur in the potato fields. In addition, no discrepancy was found in pathogenicity and noticeable changes for the appearance of variants of Phytophthora infestans in each generation when serial inoculations and the analysis of genomic DNAs by AFLP were conducted. Thus, these data suggest that transgenic potatoes do not give significant impacts on the communities of soil microorganisms and the emergence of variants, although continued research efforts may be necessary to make a decisive conclusion.
- Azevedo, J. L. and W. L. Araujo. 2003. Genetically modified crops: Environmental and human health concerns. Mutat. Res. 544: 223-233.
- Conner, A. J., T. R. Glare, and J. P. Nap. 2003. The release of genetically modified crops into the environment. Plant J. 33: 19-46.
- De la Cruiz, F. and J. Davies. 2000. Horizontal gene transfer and the origin of species: Lessons from bacteria. Trends Microbiol. 8: 128-133.
- Franklin, R. B., J. L. Garland, C. H. Bolster, and A. L. Mills. 2001. Impact of dilution on microbial community structure and functional potential: Comparison of numerical simulations and batch culture experiments. Appl. Environ. Microbiol. 67: 702-712.
- Garland, J. L. and A. L. Mills. 1991. Classification and characterization of heterotropic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl. Environ. Microbiol. 57: 2351-2359.
- Hull, R., S. N. Covey, and P. Dale. 2000. Genetic modified plants and the 35S promoter: Assessing the risks and enhancing the debate. Microb. Ecol. Health Dis. 12: 1-5.
- James, C. 2008. Global status of commercialized biotech/GM crops: 2008, ISAAA Brief No. 39. The International Service for the Acquisition of Agri-biotech Applications (ISAAA), Ithaca, NY, U.S.A.
- Kay, E., T. M. Vogel, F. Bertolla, R. Nalin, and P. Simonet. 2002. In situ transfer of antibiotics resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl. Environ. Microbiol. 68: 3345-3351.
- Kim, S. E., J. S. Moon, J. K. Kim, W. S. Choi, S. H. Lee, and S. U. Kim. 2010. Investigation of possible horizontal gene transfer from transgenic rice to soil microorganisms in paddy rice field. J. Microbiol. Biotechnol. 20: 187-192.
- Lal, R. and S. Lal. 1993. Genetic Engineering of Plants for Crop Improvement. CRC Press, Boca Raton, FL, U.S.A.
- Lewis, L. C., D. J. Bruck, R. D. Gunnarson, and K. G. Bidne. 2001. Assessment of plant pathogenicity of endophytic Beauveria bassiana in Bt transgenic and non-transgenic corn. Crop, Sci. 41: 1395-1400.
- Lu, B. R. 2006. Identifying possible environmental hazard from GM rice in China to inform biosafety assessment, pp. 106-110. In: Proceedings of the 9th International Symposium on the Biosafety of Genetically Modified Organisms, Jeju Island, Korea.
- Nielsen, K. M., F. Gebhard, K. Smalla, A. M. Bones, and J. D. van Elass. 1997. Evaluation of possible horizontal gene transfer from transgenic plants to the soil bacterium Acinetobacter calcoaceticus BD413. Theor. Appl. Genet. 95: 815-821.
- Ochman, H., J. G. Lawrence, and E. A. Groisman. 2000. Lateral gene transfer and the nature of bacterial innovation. Nature 405: 299-304.
- Rohlf, F. J. 2000. NTSYSpc v2.1. Exeter Software, Setauket, NY, U.S.A.
- Schlutter, K., J. Flutter, and I. Potrykus. 1995. Horizontal gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs, if at all, at extremely low frequency. Biol. Technol. 13: 12094-12098.
- Tang, L., S. Y. Kwon, D. J. Yun, S. S. Kwak, and H. S. Lee. 2004. Selection of transgenic potato plants expressing NDP kinase 2 gene with enhanced tolerance to oxidative stress. Korean J. Plant Biotechnol. 31: 191-195.
- Tepfer, M. 1993. Viral genes and transgenic plants. Biotechnology 11: 1125-1131.
Ye, X., S. Al-Babili, A. Kloti, J. Zhang, P. Lucca, P. Beyer, and I. Potrykus. 2000. Engineering the provitamin A (
$\beta$-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287: 303-305.
- Gebhard, F. and K. Smalla. 1999. Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbiol. Ecol. 28: 261-271.
이 논문을 인용한 문헌 (1)
- 2012. "" Journal of microbiology and biotechnology, 22(4): 563~566
- NDSL :
- 한국미생물.생명공학회 : 저널
- KCI :
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기