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Journal of microbiology and biotechnology v.20 no.6, 2010년, pp.1027 - 1031   SCIE 피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Monitoring of Possible Horizontal Gene Transfer from Transgenic Potatoes to Soil Microorganisms in the Potato Fields and the Emergence of Variants in Phytophthora infestans

Kim, Sung-Eun    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology   ); Moon, Jae-Sun    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology   ); Kim, Jung-Kyu    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology   ); Yoo, Ran-Hee    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology   ); Choi, Won-Sik    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology   ); Lee, Eun-Na    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology   ); Lee, Sang-Han    (Department of Food Science and Technology, Kyungpook National University   ); Kim, Sung-Uk    (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology  );
  • 초록

    To examine the possibility of horizontal gene transfer between transgenic potatoes and microorganisms in potato fields, the gene flow from transgenic potatoes containing the nucleoside diphosphate kinase 2 (NDPK2) gene to microorganisms in soils was investigated. The soil samples collected from the potato fields from March to October 2007 were examined by PCR, Southern hybridization, and AFLP fingerprinting. The NDPK2 gene from soil genomic DNAs was not detected by both PCR and Southern hybridization, indicating that gene transfer did not occur in the potato fields. In addition, no discrepancy was found in pathogenicity and noticeable changes for the appearance of variants of Phytophthora infestans in each generation when serial inoculations and the analysis of genomic DNAs by AFLP were conducted. Thus, these data suggest that transgenic potatoes do not give significant impacts on the communities of soil microorganisms and the emergence of variants, although continued research efforts may be necessary to make a decisive conclusion.


  • 주제어

    AFLP .   emerging variants .   horizontal gene transfer .   soil microorganisms .   transgenic NDPK2 potatoes.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2012. "" Journal of microbiology and biotechnology, 22(4): 563~566     

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