Preparation and Characterization of Silk Fibroin Nanoparticles
Silk fibroin was conjugated with methoxypoly(ethylene glycol) derivatives to prepare silk nanoparicles. Conjugation of SF with PEG was examined with various instrumental analyses. Nuclear magnetic resonance spectrometry and amino acid analysis showed that serine and tyrosine residues in SF were reacted with PEG and resulted in increasing molecular weight. The sizes and shapes of SF nanoparticles observed by transmission electronmicroscope were ranged about 150-400 nm in diameter and spherical morphology. UV/VIS spectrometry showed SF nanoparticles might be outer PEG and inner SF structure.
- Gotoh Y, Tsukada M, Minoura N (1993) Chemical modification of silk fibroin with cyanuric chloride activated poly(ethylene glycol): Analyses of reaction site by 1H-NMR spectroscopy and conformation of the conjugates. Bioconjugate Chem 4, 554-559.
- Hanawa T, Watanabe A, Tsuchiya T, Ikoma R, Hidaka M, Sugihara M (1995) New oral dosage form for elderly patients. II. Release behavior of benfotiamine from silk fibroin gel. Chem Pharm Bull, 43, 872-876.
- Harris JM (1992) Poly(Ethylene Glycol) Chemistry: Biotechnical and Biomedical Applications. Plenum, New York.
- Harris JM, Zalipsky S. (1997) Poly(ethylene Glycol): Chemistry and Biological Applications. ACS Books, Washington, D.C.
- Jackman LM, Sternhell S (1969) Theory of chemical shift. In: Applications of nuclear magnetic resonance spectroscopy in organic chemistry. 2nd ed. Oxford: Pergamon Press pp.61-113.
- Kweon H, Ha HC, Um IC, Park YH (2001) Physical Properties of Silk Fibroin/Chitosan
- Blend Films. J Appl Polym Sci 80, 928-934.
- Minoura N, Tsukada M, Nagura M (1990) Fine Structure and Oxygen Permeability of Silk
- Fibroin Membrane treated with Methanol. Polymer, 31, 265-269.
- Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 29, 1215-1221.
- Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen-I Gakkaishi, 45, 487.
- Sofia S, McCarthy MB, Gronowicz G, Kaplan DL (2001) Functionalized silk-based biomaterials for bone formation. J Biomed Mater Res 54, 139-148.
- Tan ML, Choong PFM, Dass CR (2010) Recent developments in liposomes, microparticles and nanoparticles for protein and peptide drug delivery. Peptides 31, 184-193.
- Tanford C, Hauenstein JD (1956) Hydrogen ion equilibria of ribonuclease. J Am Chem Soc 78, 5287-5291.
- Vallee BL, Riordan JF (1969) Chemical approaches to the properties of active sites of enzyme. Annu Rev Biochem 38, 733-793.
- Yamada H, Igarashi Y, Takasu Y, Saito H, Tsubouchi K (2004) Identification of fibroin-derived peptides enhancing the proliferation of cultured human skin fibroblasts. Biomaterials 25, 467-472.
- Yeo JH, Lee KG, Lee HS, Lee YW, Kim SY (2000) Studies on PVA/Chitosan/Fibroin Blend Sponge Sheets: Preparation and Wound Healing Effects in Rats. Int J Indust Entomol 1, 59.
- Cho KY, Moon JY, Lee YW, Lee KG, Yeo JH, Kweon HY, Kim KH, Cho CS (2003) Preparation of self-assembled silk sericin nanoparticles. Int J Biol Macromol 32, 36-42.
- Khvedelidze M, Mdzinarashvili T, Partskhaladze T, Nafee N, Schaefer UF, Lehr CM, Schneider M (2010) Calorimetric and spectrophotometric investigation of PLGA nanoparticles and their complex with DNA. J Therm Anal Calorim 99, 337-348.
- Nucci ML, Shorr R, Abuchowski A (1991) The therapeutic value of poly(ethylene glycol) modified proteins. Adv Drug Del Rev 6, 133-151.
이 논문을 인용한 문헌 (2)
- 2010. "" International journal of industrial entomology, 20(2): 87~91
- Kim, Sung-Kuk ; Jo, You-Young ; Lee, Kwang-Gill ; Kim, Kee-Young ; Kim, Hyun-bok ; Kweon, HaeYong 2015. "Effect of ethanol concentration on the infrared spectroscopic characteristics of silk beads" 한국잠사곤충학회지 = Journal of sericultural and entomological science, 53(2): 118~123
- 한국학술정보 : 저널
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기