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No Genetic Differentiation of Elaphe schrenckii Subspecies in Korea Based on 9 Microsatellite Loci

An, Jung-Hwa    (Research Institute for Veterinary Science, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University   ); Park, Dae-Sik    (Division of Science Education, Kangwon National University   ); Lee, Jung-Hyun    (Department of Biology, Kangwon National University   ); Kim, Kyung-Seok    (Research Institute for Veterinary Science, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University   ); Lee, Hang    (Conservation Genome Resource Bank for Korean Wildlife (CGRB)   ); Min, Mi-Sook    (Conservation Genome Resource Bank for Korean Wildlife (CGRB)  );
  • 초록

    The Russian ratsnake, Elaphe schrenckii, is found in Russia, China, and Korea, and is considered to be an endangered species by the Ministry of Environment in South Korea. Due to habitat loss and use in oriental medicine, their population has been severely decimated. In South Korea, two subspecies of E. schrenckii has been defined according to body color: E. s. schrenckii (blackish) and E. s. anomala (yellow-brownish). Molecular genetic studies on Elaphe schrenckii are very scarce and the taxonomy of Elaphe schrenckii subspecies is uncertain. From the present study, we attempted to identify the genetic differences of these two subspecies using species-specific microsatellites developed from the genomic library of E. schrenckii. Nine polymorphic loci were tested on 19 individuals from E. s. schrenckii (n=10) and E. s. anomala (n=9) in South Korea. The mean number of alleles was 3.78 in E. s. schrenckii and 4.11 in E. s. anomala. The average expected heterozygosity was 0.542 and 0.511 in E. s. schrenckii and E. s. anomala, respectively. We found a lack of genetic structure between two subspecies ( $F_{ST}=0.016$ ) and no genetic discrimination between two subspecies was found. Based on the present findings by microsatellites, two subspecies can be considered as one species, E. schrenckii. However, further investigations on taxonomical status using mitochondrial and nuclear DNA sequences need to be performed and morphological & ecological data should be revised. The genetic markers should benefit future studies of the endangered species of other Elaphe species for the study of genetic diversity and potential conservation management.

  • 주제어

    Colubridae .   Elaphe schrenckii schrenckii .   Elaphe schrenckii anomala .   microsatellite .   genetic diversity.  

  • 참고문헌 (28)

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