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International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute v.35 no.1, 2010년, pp.1 - 5  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Effects of Knockout Serum Replacement in the Culture Medium on the Proliferation of Porcine Fetal Fibroblasts In Vitro

Kim, Eun-Ju    (Cellular Reprogramming and Embryo Biotechnology Lab., Department of Cancer and Developmental Biology, Dental Research Institute, and CLS21, Seoul National University School of Dentistry   ); Park, Jung-Joo    (University of Pennsylvania School of Dental Medicine   ); Choi, Young-Ju    (Cellular Reprogramming and Embryo Biotechnology Lab., Department of Cancer and Developmental Biology, Dental Research Institute, and CLS21, Seoul National University School of Dentistry   ); Park, Sang Kyu    (Cellular Reprogramming and Embryo Biotechnology Lab., Department of Cancer and Developmental Biology, Dental Research Institute, and CLS21, Seoul National University School of Dentistry   ); Roh, Sang-Ho    (Cellular Reprogramming and Embryo Biotechnology Lab., Department of Cancer and Developmental Biology, Dental Research Institute, and CLS21, Seoul National University School of Dentistry  );
  • 초록

    Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum. However, there are potential disadvantages of the use of serum including unnatural interactions between the cells and the potential for exposure to animal pathogens. To prevent the possible influence of serum on fibroblast cultures, we devised a serum-free growth method and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. These cells were grown under four different culture conditions: no serum (negative control), 10% fetal bovine serum (FBS, positive control), 10% knockout serum replacement (KSR) and 20% KSR in the medium. The proliferation rates and viabilities of the cells were investigated by counting the number of cells and trypan blue staining, respectively. The 10% FBS group showed the largest increase in the total number of cells ( $1.09\;{\times}\;10^5\;cells/ml$ ). In terms of the rate of viable cells, the results from the KSR supplementation groups (20% KSR:64.7%; 10% KSR: 80.6%) were similar to those from the 10% FBS group (68.5%). Moreover, supplementation with either 10% ( $3.0\;{\times}\;10^4\;cells/ml$ ) or 20% KSR ( $4.8\;{\times}\;10^4\;cells/ml$ ) produced similar cell growth rates. In conclusion, although KSR supplementation produces a lower cell proliferation rate than FBS, this growth condition is more effective for obtaining an appropriate number of viable porcine fetal fibroblasts in culture. Using KSR in fibroblast culture medium is thus a viable alternative to FBS.


  • 주제어

    fibroblast .   serum replacement .   serum-free culture.  

  • 참고문헌 (18)

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