본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of advanced prosthodontics v.2 no.1, 2010년, pp.18 - 24   피인용횟수: 1

Effect of etched microgrooves on hydrophilicity of titanium and osteoblast responses: A pilot study

Park, Jung-Ae    (Department of Prosthodontics, Graduate School of Dentistry   ); Lee, Sung-Bok Richard    (Department of Biomaterials & Prosthodontics, East-West Neo Medical Center, Institute of Oral Biology, School of Dentistry, Kyung Hee University   ); Ahn, Su-Jin    (Department of Biomaterials & Prosthodontics, East-West Neo Medical Center, Institute of Oral Biology, School of Dentistry, Kyung Hee University   ); Lee, Suk-Won    (Department of Biomaterials & Prosthodontics, East-West Neo Medical Center, Institute of Oral Biology, School of Dentistry, Kyung Hee University  );
  • 초록

    PURPOSE. The aim of this pilot study was to investigate the effect of etched microgrooves on the hydrophilicity of Ti and osteoblast responses. MATERIAL AND METHODS. Microgrooves were applied on Ti to have 15 and $60{\mu}m$ width, and 3.5 and $10{\mu}m$ depth by photolithography, respectively. Further acid etching was applied to create Ti surfaces with etched microgrooves. Both smooth- and acid-etched Ti were used as the controls. The hydrophilicity of Ti was analyzed by determining contact angles. Cell proliferation and osteogenic activity of MC3T3 mouse preosteoblasts were analyzed by bromodeoxyuridine assay and alkaline phosphatase (ALP) activity test, respectively. One-way ANOVA, Pearson's correlation analysis and multiple regression analysis were used for statistics. RESULTS. Etched microgrooves significantly increased the hydrophilicity of Ti compared to the smooth Ti. $60{\mu}m$ -wide etched microgrooves significantly enhanced cell proliferation, whereas the osteogenic activity showed statistically non-significant differences between groups. Result of the osteogenic activity significantly correlated with those of hydrophilicity and cell proliferation. Hydrophilicity was determined to be an influential factor on osteogenic activity. CONCLUSION. This study indicates that increase in hydrophilicity of Ti caused by etched microgrooves acts as an influential factor on osteogenic activity. However, statistically non-significant increase in the ALP activity suggests further investigation.

  • 주제어

    Titanium .   Etched microgrooves .   Hydrophilicity .   Osteoblast response.  

  • 참고문헌 (18)

    1. den Braber ET, de Ruijter JE, Ginsel LA, von Recum AF, Jansen JA. Quantitative analysis of fibroblast morphology on microgrooved surfaces with various groove and ridge dimensions. Biomaterials 1996;17:2037-44. 
    2. Chou L, Firth JD, Uitto VJ, Brunette DM. Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts. J Cell Sci 1995;108:1563-73. 
    3. Hamilton DW, Wong KS, Brunette DM. Microfabricated discontinuous-edge surface topographies influence osteoblast adhesion, migration, cytoskeletal organization, and proliferation and enhance matrix and mineral deposition in vitro. Calcif Tissue Int 2006;78:314-25. 
    4. Hamilton DW, Brunette DM. The effect of substratum topography on osteoblast adhesion mediated signal transduction and phosphorylation. Biomaterials 2007;28:1806-19. 
    5. Kenar H, Kose GT, Hasirci V. Tissue engineering of bone on micropatterned biodegradable polyester films. Biomaterials 2006;27:885-95. 
    6. Chehroudi B, Ratkay J, Brunette DM. The role of implant surface geometry on mineralization in vivo and in vitro; a transmission and scanning electron microscopic study. Cells and Materials 1992;2:89-104. 
    7. Qu J, Chehroudi B, Brunette DM. The use of micromachined surfaces to investigate the cell behavioural factors essential to osseointegration. Oral Dis 1996;2:102-15. 
    8. Perizzolo D, Lacefield WR, Brunette DM. Interaction between topography and coating in the formation of bone nodules in culture for hydroxyapatite- and titanium-coated micromachined surfaces. J Biomed Mater Res 2001;56:494-503. 
    9. Lamolle SF, Monjo M, Rubert M, Haugen HJ, Lyngstadaas SP, Ellingsen JE. The effect of hydrofluoric acid treatment of titanium surface on nanostructural and chemical changes and the growth of MC3T3-E1 cells. Biomaterials 2009;30:736-42. 
    10. Schwarz F, Ferrari D, Herten M, Mihatovic I, Wieland M, Sager M, Becker J. Effects of surface hydrophilicity and microtopography on early stages of soft and hard tissue integration at non-submerged titanium implants: an immunohistochemical study in dogs. J Periodontol 2007;78:2171-84. 
    11. Schwarz F, Wieland M, Schwartz Z, Zhao G, Rupp F, Geis-Gerstorfer J, Schedle A, Broggini N, Bornstein MM, Buser D, Ferguson SJ, Becker J, Boyan BD, Cochran DL. Potential of chemically modified hydrophilic surface characteristics to support tissue integration of titanium dental implants. J Biomed Mater Res B Appl Biomater 2009;88:544-57. 
    12. Lee SW, Kim SY, Rhyu IC, Chung WY, Leesungbok R, Lee KW. Influence of microgroove dimension on cell behavior of human gingival fibroblasts cultured on titanium substrata. Clin Oral Implants Res 2009;20:56-66. 
    13. Kim SY, Oh N, Lee MH, Kim SE, Leesungbok R, Lee SW. Surface microgrooves and acid etching on titanium substrata alter various cell behaviors of cultured human gingival fibroblasts. Clin Oral Implants Res 2009;20:262-72. 
    14. Zhao Y, Lu Q, Li M, Li X. Anisotropic wetting characteristics on submicrometer-scale periodic grooved surface. Langmuir 2007;23:6212-7. 
    15. Walboomers XF, Ginsel LA, Jansen JA. Early spreading events of fibroblasts on microgrooved substrates. J Biomed Mater Res 2000;51:529-34. 
    16. Matsuzaka K, Walboomers XF, de Ruijter JE, Jansen JA. The effect of poly-L-lactic acid with parallel surface micro groove on osteoblast-like cells in vitro. Biomaterials 1999;20:1293-301. 
    17. Lee SW, Kim SY, Lee MH, Lee KW, Leesungbok R, Oh N. Influence of etched microgrooves of uniform dimension on in vitro responses of human gingival fibroblasts. Clin Oral Implants Res 2009;20:458-66. 
    18. Ismail FS, Rohanizadeh R, Atwa S, Mason RS, Ruys AJ, Martin PJ, Bendavid A. The influence of surface chemistry and topography on the contact guidance of MG63 osteoblast cells. J Mater Sci Mater Med 2007;18:705-14. 
  • 이 논문을 인용한 문헌 (1)

    1. 2015. "" The journal of advanced prosthodontics, 7(6): 496~505     

 저자의 다른 논문

  • Ahn, Su Jin (13)

    1. 2001 "수직적 교합고경의 증가가 사지 근력에 미치는 영향에 관한 연구" 대한치과턱관절기능교합학회지 = Journal of Korean academy of stomatognathic function and occlusion 17 (4): 231~244    
    2. 2010 "교합안정장치가 전문골프선수들의 드라이버 비거리에 미치는 영향" 구강회복응용과학지 = Journal of dental rehabilitation and applied science 26 (2): 157~168    
    3. 2010 "수직적 교합고경의 증가가 사지 근력에 미치는 영향에 관한 연구" 구강회복응용과학지 = Journal of dental rehabilitation and applied science 26 (2): 169~178    
    4. 2011 "지르코니아의 표면처리 방법에 따른 압축강화형 복합레진 ($TESCERA^{TM}$ ATL)전장의 결합강도" 구강회복응용과학지 = Journal of dental rehabilitation and applied science 27 (1): 1~13    
    5. 2015 "복합적인 전신질환이 있는 안면마비 환자의 양악 자성 피개의치 수복 증례" 대한치과보철학회지 = The journal of Korean academy of prosthodontics 53 (2): 150~156    
    6. 2016 "Influence of the connection design and titanium grades of the implant complex on resistance under static loading" The journal of advanced prosthodontics 8 (5): 388~395    
    7. 2017 "엇갈린 교합에서 implant와 magnetic attachment를 이용한 국소의치 증례" 대한치과보철학회지 = The journal of Korean academy of prosthodontics 55 (1): 53~60    
    8. 2017 "시멘트 유지형 임플란트 보철물의 신개념 유지 방식: Fiber post의 설측 수평 삽입에 의한 유지력 보완" 대한치과보철학회지 = The journal of Korean academy of prosthodontics 55 (1): 71~78    
    9. 2017 "Analysis of crown size and morphology, and gingival shape in the maxillary anterior dentition in Korean young adults" The journal of advanced prosthodontics 9 (4): 315~320    
  • Lee, Suk-Won (15)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기