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Journal of ginseng research = 高麗人參學會誌 v.34 no.2, 2010년, pp.113 - 121   피인용횟수: 4
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Physicochemical Characterization and NMR Assignments of Ginsenosides Rb1, Rb2, Rc, and Rd Isolated from Panax ginseng

Cho, Jin-Gyeong    (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University   ); Lee, Min-Kyung    (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University   ); Lee, Jae-Woong    (Department of Horticultural Biotechnology, Kyung Hee University   ); Park, Hee-Jung    (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University   ); Lee, Dae-Young    (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University   ); Lee, Youn-Hyung    (Department of Horticultural Biotechnology, Kyung Hee University   ); Yang, Deok-Chun    (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University   ); Baek, Nam-In    (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University  );
  • 초록

    The fresh ginseng roots were extracted with aqueous methanol, and the obtained extracts were partitioned using ethyl acetate, n-butanol, and water, successively. The repeated silica gel and octadecyl silica gel column chromatogaraphy for n-butanol fraction afforded four diol ginseng saponins, ginsenosides $Rb_1$ , $Rb_2$ , $R_c$ , and Rd. The physicochemical, spectroscopic, and chromatographic characteristics of these ginsenosides were measured and compared with those reported in the literature. Some of the peak assignments in previously published $^1H$ - and $^{13}C$ -nuclear magnetic resonance (NMR) spectra were inaccurate. This study employed two-dimensional NMR experiments, including $^1H-^1H$ correlation spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity, to determine exact peak assignments.


  • 주제어

    Diol ginsenoside .   Fast atom bombardment/mass spectrometry .   High-performance liquid chromatography .   Nuclear magnetic resonance.  

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  • 이 논문을 인용한 문헌 (4)

    1. 2011. "" Journal of ginseng research = 高麗人參學會誌, 35(1): 31~38     
    2. 2011. "" Journal of ginseng research = 高麗人參學會誌, 35(1): 111~123     
    3. Yang, Seung Ok ; Lee, Sung Woo ; Kim, Young Ock ; Lee, Sang Won ; Kim, Na Hyun ; Choi, Hyung Kyoon ; Jung, Joo Yeoun ; Lee, Dong Ho ; Shin, Yu Su 2014. "Comparative Analysis of Metabolites in Roots of Panax ginseng Obtained from Different Sowing Methods" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 22(1): 17~22     
    4. 2015. "" Natural product sciences, 21(2): 111~121     

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