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한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology v.38 no.2, 2010년, pp.222 - 226   피인용횟수: 3
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김치로부터 담즙산 분해능이 우수한 Lactobacillus plantarum CIB 001의 분리 및 동정
Isolation and Identification of Lactobacillus plantarum CIB 001 with Bile Salt Deconjugation Activity from Kimchi

차상도    ((주)셀인바이오 생물소재연구소   ); 김태운    (경희대학교 식품공학과   ); 이동희    ((주)셀인바이오 생물소재연구소  );
  • 초록

    This study was carried out to isolate and characterize the Lactobacillus plantarum with bile salt deconjugation activity that was isolated from Kimchi. Some isolates were selected and identified as L. plantarum by 16S rRNA gene sequence and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell protein patterns. They were assayed to determine their capacities to express bile salt hydrolase (BSH) activity. Among the identified strains, L. plantarum CIB 001 showed the highest level of BSH activity. Then, resistance to gastric acidity and bile condition were analyzed for further characterization. This strain was able to maintain viability for 1h at pH 2.0 and to survive in a MRS (deMan, Rogosa, and Sharpe) broth with 1.0% of bile acids. L. plantarum CIB 001 would potentially be useful in the food industry as probiotics.


  • 주제어

    Lactobacillus plantarum .   bile salt hydrolase.  

  • 참고문헌 (30)

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  • 이 논문을 인용한 문헌 (3)

    1. Jeong, Ji-Kang ; Zheng, Yanfei ; Choi, Hye-Sun ; Han, Gwi-Jung ; Park, Kun-Young 2010. "Catabolic Enzyme Activities and Physiological Functionalities of Lactic Acid Bacteria Isolated from Korean Traditional Meju" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(12): 1854~1859     
    2. Jung, Gwangsick ; Lee, Jong-Hoon 2012. "Evaluation of the Colonization of Lactobacillus plantarum in Mouse Gut by Terminal Restriction Fragment Length Polymorphism Analysis" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 40(4): 389~395     
    3. Lee, Jae-Joon ; Kim, Ah-Ra ; Chang, Hae-Choon ; Lee, Myung-Yul 2012. "Repeated-Dose Oral Toxicity Study of Lactobacillus Plantarum AF1 Isolated from Kimchi in Rats" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(5): 612~620     

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