본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Ocean and polar research v.32 no.2, 2010년, pp.157 - 164   피인용횟수: 2
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Spirulina maxima 초고압 추출물의 피부 면역 활성 증진
Enhancement of Skin Immune Activities of Spirulina maxima by High Pressure Extraction Process

오성호    (강원대학교 바이오산업공학부   ); 강도형    (한국해양연구원 해양생물자원 연구부   ); 최운용    (강원대학교 바이오산업공학부   ); 서용창    (강원대학교 바이오산업공학부   ); 허수진    (한국해양연구원 해양생물자원 연구부   ); Abu, Affan Md.    (한국해양연구원 해양생물자원 연구부   ); 정경환    (충주대학교 보건생명대학 식품생명공학부   ); 이현용    (강원대학교 바이오산업공학부  );
  • 초록

    A marine alga, Spirulina maxima, was extracted under high pressure and low temperature conditions at 500 MPa and $60^{\circ}C$ for 5 and 10 min. A high pressure of 500 MPa was applied to improve process yields because of low temperature extraction. This method resulted in highest higher extraction yield of 26.1% (w/w) in comparison to those results obtained from conventional extraction methods which produced a yield of 17.6% (w/w) from water. The extracts from this process also showed 19% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. The crude extract significantly reduced the production of Prostaglandin $E_2$ ( $PGE_2$ ) from CCD-986sk cells and increased nitric oxide production by macrophages. These higher activities of enhancing skin immune functions were found to have high antioxidant extract properties, like a 98% increase in DPPH radical scavenging activity. The extracts from the high pressure process showed a higher elution of active components than other processes and generated new compounds based on HPLC analysis. This clearly indicates that the extracts from high pressure and low temperature conditions have higher skin immune activation properties that have not been previously reported.


  • 주제어

    Spirulina maxima .   skin immune activities .   high pressure .   low temperature .   antioxidant.  

  • 참고문헌 (25)

    1. 강민숙, 심상준, 채희정 (2004) 기능성 생물 소재로서의 클로렐라. 한국생물공학회지 19(1):1-11     
    2. 이윤진, 손찬욱, 김혜정, 이진하, 김미리 (2009) 스피루리나 첨가한 생면과 조리면의 저장 중 품질특성. 한국식품저장유통학회지 16(1):23-32     
    3. Bernstein EF, Chen YQ (1994) Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J Invest Dermatol 103(2):182-186 
    4. Choi SY, Hwang JS, Kim S, Kim SY (2006) Synthesis, discovery and mechanism of 2,6-dimethoxy-N-(4-methoxyphenyl)benzamide as potent depigmenting agent in the skin. Biochem Bioph Res Co 349(1):39-49 
    5. Chung TY, Kim MA, Jones AD (1996) Antioxidative activity of flavonoids isolated from jindalrae flower. Plant Physiol Bioch 39(4):320-326 
    6. Diego JM, Gomez C, Ibanez E, Ruperez FJ, Barbas C (2004) Tocopherol measurement in edible products of vegetable origin. J Chromatogr A 1054(1/2):227-233 
    7. Grinstead GS, Tokach SS, Goodband RD, Nelssen JL (2000) Effects of Spifulina Platensis in growth performance of weanling pigs. Anim Feed Sci Tech 83(3-4):237-247 
    8. Hernandez AC, Nieves I, Meckes M, Chamorro G, Barron BL (2002) Antiviral activity of Spirulina maxima against herpes simplex virus type 2. Antivir Res 56(3):297-285 
    9. Jeong DM, Kim HM, Kim JM (2002) Antioxidative treatment method by lxeris dentata for conquer cancer and stress. J Korean Soc Jungshin Sci, 6(1):69-82 
    10. Jeong HS, Kwon MC, Han JG, Ha JH, Jin L, Kim JC, Kwak HG, Hwang BY, Lee HY (2008) Enhancement of skin immune activation effect of collagen peptides isolated from Asterias amurensis. Korean J Food Sci Ttecnol 40(5):522-527     
    11. Jeong MH, Kim SS, Ha JH, Jin L, Lee HJ, Kang HY, Park SJ, Lee HY (2009) Enhancement of anticancer activity of acer mono by high pressure extraction process. J Korean Soc Food Sci Nutr 38(9):1243-1252     
    12. Jeong MH, Ha JH, Oh SH, Kim SS, Lee HJ, Kang HY, Lee HY (2009) Comparison of biological activities of Acer mono and A. okamotoanum extracts by water extraction and low temperature high pressure extraction. Korean J Med Crop Sci 7(6):407-414     
    13. Jin L, Ha JH, Jeong MH, Chung EK, Chung AR, Kim JC, Ahn JH, Lee HY (2009) Enhancement of the antioxidant and anticancer activities of Berberis koreana bark by using a low temperature and high-pressure extraction process. Korean J Food Sci Technol 41(3):284-291     
    14. Jose C, Pierre VA, Lenka T, Gabriela O, Doris V, Karim ZB, Michel V, Jean N (2010) Free radical-scavenging, antioxidant and immunostimulating effects of a licorice infusion. Food Chem. 122(3):508-517. doi:10.1016/j.foodchem.2010.02.060 
    15. Jung SJ, Lee JH, Song HN, Seong NS, Lee SE, Baek NI (2004) Screening for antioxidant activity of plant medicinal extracts. J Korean Soc Appl Biol Chem 47(1):135-140     
    16. Kaji T, Fujiwara Y, Inomata Y, Hamada C, Yamamoto C, Shimada S, Lee JB, Hayashi T (2002) Repair of wounded monolayers of cultured bovine aortic endothelial cells is inhibited by calcium spirulan, a novel sulfated polysaccharide isolated form Spirulina platensis. Life Sci 70(16):1841-1848 
    17. Kay RA (1991) Microalgae as food and supplement. Crit Rev Food Sci 30(6):555-573 
    18. Oh SH, Jeoung MH, Kim SS, Ahn JH, Kang DH, Lee HY (2010) The effect of ultrasonificated extracts of Spirulina maxima on the anticancer activity. Mar Biotechnol. doi: 10.1007/s10126-010-9282-2 
    19. Lee HH, Lee SY (2008) Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai and T. officinale WEB. extracts. Korean J Med Crop Sci 16(2):79-85     
    20. Lee HS, Lee SH, Mun HC, Lee HY (2003) Screening of the immuno-stimulatory activity of the marine alga Chlorella capsulate. Kor J Biotechnol Bioeng 18(1):19-24     
    21. Liou JY, Ellent DP, Lee S, Goldsby J, Ko BS, Matijevic N, Huang JC, Wu KK (2007) Cyclooxygenase-2-derived prostaglandin e2 protects mouse embryonic stem cells from apoptosis. Stem Cells 25(5):1096-1103 
    22. Mosmann T (1983) Rapid colorimetric assay for the cellular growth and survival: Application to proliferation and cytotoxic assay. J Immunol Methods 65(1/2):55-63 
    23. Nandeesha MC, Gangadhara B, Manissery KK, Venkataraman LV (2001) Growth performance of two indian major carps, catla fed diets containing different levels of Spirulina platensis. Bioresour Technol 80(2):117-120 
    24. Park JH, Oh SM, Lim SS, Lee YS, Shin HK, Oh YS, Choe NH, Park JHY, Kim JK (2006) Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages. Biochem Biophys Res Commun 351(1):146-152 
    25. Yang HN, Lee EH, Kim HM (1997) Spirulina platensis inhibits anaphylactic reactin. Life Sci 61(13):1237-1244 
  • 이 논문을 인용한 문헌 (2)

    1. Ling, Jin ; Kim, Ji-Seon ; Seo, Yong-Chang ; Choi, Woon-Yong ; Ahn, Ju-Hee ; Ma, Choong-Je ; Yoon, Chang-Soon ; Lee, Hyeon-Yong 2011. "The Effect of Extracts of Berberis koreana Bark by Lactobacillus Fermentation on the Concentration of Serotonin and Melatonin in the Serum of Treated ICR Mice" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 19(2): 117~125     
    2. Kim, Nam Young ; Lee, Hyeon Yong 2014. "Enhancement of Skin Immune Activities of Curcuma longa L. Leaf Extract by Ultra High Pressure Process" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 22(5): 378~383     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기