본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition v.39 no.6, 2010년, pp.796 - 804   피인용횟수: 11
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

뽕잎발효차 제조에 따른 항산화 및 in vitro 항암활성
Antioxidant Activity and in vitro for Anticancer Effects of Manufactured Fermented Mulberry Leaf Tea

배만종    (대구한의대학교 한방식품약리학과   ); 예은주    ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원  );
  • 초록

    본 연구는 식용식물로의 가치뿐만 아니라 약용으로 가치가 인정되는 뽕잎에 미생물 생균제를 이용한 뽕잎발효차 개발을 통하여 기능성 소재 개발 및 새로운 제품개발의 방안을 제시하고자 뽕잎차 및 뽕잎발효차의 여러 추출물의 항산화성 및 in vitro에서의 항암활성을 비교분석 하였다. 뽕잎차 및 뽕잎발효차의 각 추출물에 있어 항산화 효과를 조사한 결과 각 추출물의 농도와 비례하여 전자공여활성이 증가하였고 대체로 뽕잎발효차 추출물이 뽕잎차의 추출물보다 전자공여활성이 더 높은 것으로 나타났으며, 각 추출물의 종류 중 에탄올 추출물의 전자공여능이 가장 높은 것으로 분석되었다. SOD 유사활성도를 조사한 결과 각 추출물의 농도와 비례하여 SOD 유사활성도는 증가하는 경향이었고, 추출물중 에탄올 추출물군의 활성도가 가장 높았으며 두 시료의 SOD 유사활성도는 비슷하였다. 각 pH별 아질산염 분해 작용을 분석한 결과 pH 1.2에서 아질산염 분해 작용이 가장 컸으며, 추출물 군 중에서 에탄올 추출물 군이 아질산염 소거능이 가장 높았다. 뽕잎차 및 뽕잎발효차 각 추출물의 in vitro 상에서 항암효과를 분석한 결과 HeLa cell과 MCF-7 cell 모두 뽕잎발효차가 뽕잎차보다 암세포 증식 억제율이 더 높았다. 추출물 군에서는 에탄올 추출물군이 암세포 성장을 가장 많이 억제하였고 MCF-7 cell이 HeLa cell에서 보다 더 효과적인 것으로 나타났다. 따라서 본 연구의 결과를 종합해 보면 식용식물로서 뿐만 아니라 약용으로도 가치가 있는 뽕잎에 생균제를 이용하여 개발한 뽕잎발효차를 뽕잎차와 비교하였을 때 항산화성, in vitro에서의 항암활성을 검증한 결과 생균제를 이용한 뽕잎발효차가 뽕잎차보다 우수한 것으로 나타났으며 뽕잎발효차 추출물의 기능성이 더 우수한 것은 발효를 통해 생리활성 물질이 생성된 것이 원인으로 사료되며 이 점에 있어 구체적인 연구가 필요하다고 판단된다. 이러한 점을 보완하여 제품개발에 응용하고 대중적인 면을 좀 더 개선한다면 새로운 기능성소재 및 제품이 될 것 이라 사료된다.


    This study was conducted to compare and analyze the qualitative property of MLT (mulberry leaf tea) and FMLT (fermented mulberry leaf tea) based on the antioxidant, anticancer activities of various extracts. When the antioxidant activity of MLT and FMLT extracts was evaluated, the electron donating activity was found to increase proportionally as the concentration of each extract increased. In addition, the extract of FMLT showed a higher electron donating activity than that of MLT. Furthermore, the ethanol extracts showed the highest electron donating ability. When the SOD activity was evaluated, it was also found to increase proportionally with the concentration of each extract. Furthermore, the SOD activity of the ethanol extract group was the highest, whereas the SOD like activities of both MLT and FMLT were similar. When nitrite decomposition was evaluated for each pH, the highest value was observed at pH 1.2. Finally, the nitrite deleting ability was the highest for the ethanol extracts. When each extract of MLT and FMLT was analyzed in vitro for anticancer effects, they were found to decrease the number of cancer cells proportionally as the concentration of extract increased for both HeLa cells and MCF-7 cells. Furthermore, FMLT was found to exert a greater inhibition of cancer cells than MLT. Among the extract groups, the ethanol extract induced the greatest inhibition of the development of cancer cells, and these effects were greater against MCF-7 cells than HeLa cells.


  • 주제어

    fermentation .   mulberry leaf .   tea .   antioxidant .   anticancer.  

  • 참고문헌 (49)

    1. Cha YJ, Lee JW, Kim JH, Park MH, Lee SY. 2004. Major components of teas manufactured with leaf and flower of Korean native Camellia japonica L. Korean J MedicinalCrop Sci 12: 183-190.     
    2. Tsuda T, Watanabe M, Ohshima K, Norinobu S, Choi SW,Kawakishi S, Osawa T. 1994. Antioxidative activity of the anthocyanin pigments cyanidin 3-ο-$\beta$-D-glucoside and cyanidin. J Agic Food Chem 42: 2407-2410. 
    3. Hatano T. 1995. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Nat Med 49: 357-363. 
    4. Akaike T, Ijiri S, Sato K, Maeda H. 1995. Determination of peroxyl radical-scavenging activity in food by using bactericidal action of alkyl peroxyl radical. J Agric Food Chem 43: 1864-1870. 
    5. 박헌국, 방병호, 소명환, 손흥수, 이재우, 정수현. 2007. 식품미생물학. 문운당, 서울. p 222-308. 
    6. Lee JR, Hah YJ, Lee JW, Song YM, Jin SK, Kim IS, HahKH, Kwak SJ. 2002. Physico-chemical and sensory properties of emulsified sausages containing mulberry and persimmon leaf powder. Korean J Food Sci Ani Resour 22:330-336.     
    7. Kim HB, Kim AJ, Kim SY. 2003. The analysis of functional materials in mulberry fruit and feed product development trends. Food Sci Industry 36: 49-60. 
    8. Naitoh K. 1968. Studies on the micro constituent in mulberry leaves part 2. Isolation of rutin and quercetin from mulberry leaves. Nippon Nogei Kagaku Kaishi 42: 422-425. 
    9. Kim JS, Kang SS, Lee MW, Kim OK. 1995. Isolation of flavonoids from the leaves of Aralia concinentails. Kor JPhamacign 26: 239-243.     
    10. Onogi A, Osawa K, Yasuda H, Sakai A, Morita H, TokawaH. 1993. Flavonol glycosides from the leaves of Morus alba.Shoyakugaku Zasshi 47: 423-425. 
    11. Makam KR. 1989. Methods in plant biochemistry in flavone.Flavonols and Their Glycosides 1: 197-235. 
    12. Lim MJ, Bae YI, Jeong CH, Cho BR, Choi JS. 2007. Phytochemical components of mulberry leaf tea by different roasting processes. J Agric Life Sci 41: 17-24. 
    13. Bang HS, Lee WC, Chon HR, Choi YC, Kim HB. 1998.Varietal comparison of $\gamma$-aminobutysic acid content inmulberry root bark. Korean J Seric Sci 40: 13-16.     
    14. Kim SY, Gao JJ, Lee WC, Ryu KS, Lee KR, Kim YC. 1999.Antioxidative flavonoids from the leaves of Morus alba.Arch Pharm Res 22: 81-85.     
    15. Omori M, Yano T, Okamoto J, Tsushida T, Murai T, HiguchiM. 1987. Effect of anaerobically treated tea (Gabaron tea) on blood pressure of spontaneously hypertensive rats.Nippon Nigeikagaki Kaishi 61: 1449-1451. 
    16. Tews JK. 1981. Dietary GABA decreases body weight of genetically obese mice. Life Science 29: 2535-2542. 
    17. Kimura M, Chen F, Nakashimqa N, Kimura I, Asano N,Koya S. 1995. Anti hyperglycemic effect of N-containing sugars delivered from mulberry leaves in streptozotocininduced diabetic mice. J Traditional Medicine 12: 214-219. 
    18. Sung GB. 1998. Recent mulberry research trend and direction for the improvement. Korean J Seric Sci 40: 180-184. 
    19. Yen GC, Wu SC, Duh PD. 1996. Extraction and identification of antioxidant components from the leaves of mulberry (Morus alba L.). J Agric Food Chem 44: 1687-1690. 
    20. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. 
    21. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem47: 469-474. 
    22. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987.Inhibition of nitrosamine formation by nondialyzable melanoidins.Agric Biol Chem 51: 1333-1338. 
    23. Park EM, Kim SJ, Ye EJ, Bae MJ, Jo KC. 2005. Effect of mycelia extracts of mushroom-cultured ginseng by-product on proliferation in cancer cell lines. J Korean Soc FoodSci Nutr 34: 323-329.     
    24. Duncan DB. 1955. Multiple range and multiple F test.Biometrics 11: 1-42. 
    25. Aoshima H, Tsunoue H, Koda H, Kiso Y. 2004. Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazl radical scavenging activity. J Agric Food Chem 52: 5240-5244. 
    26. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27: 80-85.     
    27. Shin DH. 1998. Antioxidative substances in mulberry leaves.J Korean Oil Chem Soc 16: 27-31.     
    28. Cho YJ, Ju IS, Kim BO, Kim JH, Lee BG, An BJ, ChooJW. 2007. The antimicrobial activity against Helicobacter pylori and antioxidant effect from the extracts of mulberryleaves (Morus Alba L.). J Korean Soc Appl Biol Chem50: 334-343.     
    29. An BJ, Lee JT, Lee SA, Kwak JH, Park JM, Lee JY, Son JH. 2004. Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis L. J Kor SocApple Biol Chem 47: 244-250.     
    30. Kim SD, Park BH, Back KY, Lee SI. 2008. Quality and antioxidative characteristics of Cudrania tricuspidata leavestea. Korean J Food Preserv 15: 461-468.     
    31. Hassan O, Fan LS. 2005. The anti-oxidation potential of polyphenol extract from cocoa leaves on mechanically deboned chicken meat (MDCM). LWT-Food Sci Technol 38:315-321. 
    32. Jang MJ, An BJ, Lee CE, Lee JT, Lee BG, Lee DH. 2008.Study on anti-oxidant effect of Pinus rigida Mill. inner bark extract. J Korean For Soc 97: 88-94.     
    33. Seo HS, Chung BH, Cho YG. 2008. Antioxidant and anticancer effects of Agrimony (Agrimonia pilosa L.) and Chinese Lizardtail (Saururus chinensis Baill). Korean JMedicinal Crop Sci 16: 139-143.     
    34. Lee YS, Park DC, Joo EY, Sin SL, Kim NU. 2005. Study on the antioxidant activity of the extracts from the Lepistanuda. J Korean Soc Food Sci Nutr 34: 942-947.     
    35. Yun GY, Hong JY, Nam HS, Moon YS, Sin SL. 2007.Antioxidant activities and xanthine oxidase inhibitory effectsof hot water extracts from fruits of Elaeagnus multifloraThunb. in maturity. J Korean Soc Food Sci Nutr 36:14-19.     
    36. Kim HK, Na KM, Ye SH, Hanm HS. 2004. Extraction characteristics and antioxidative activity of Schiznadra chinensisextracts. Korean J Food Culture 19: 484-490.     
    37. Choi SY, Kim JS. 2008. Physicochemical properties and antioxidative activities of Omija (Schizandra chinensis Bailon).Korean J Food & Nutr 21: 35-42.     
    38. Ra KS, Suh HJ, Chung SH, Son JY. 1997. Antioxidative activity of solvent extract onion skin. Korean J Food SciTechnol 29: 595-600.     
    39. Song ES, Park SJ, Woo NRA, Won MH, Choi JS, Kim JG,Kang MH. 2005. Antioxidant capacity of colored barley extracts by varieties. J Korean Soc Food Sci Nutr 34:1491-1497.     
    40. Cha CG, Jin WS, Kyung JM. 2008. Antioxidative and antitumor activity of extracts from Saussurea lappa. J Env Hlth Sci 34: 55-61. 
    41. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. KoreanJ Food Sci Techol 28: 232-239.     
    42. Perter FS. 1975. The toxicology of nitrate, nitrite and N-nitrocompounds.J Sci Food Agric 26: 1761-1769. 
    43. Moon JS, Kim SJ, Park YM, Hwang IS, Kim EH, Park JW,Park IB, Kim SW, Kang SG, Park YK, Jung ST. 2004.Activities of antioxidation and alcohol dehydrogenase inhibition of methanol extracts from some medicinal herbs.Korean J Food Preserv 11: 201-206.     
    44. Fiddler W, Pensabene JW, Piotrowski EG, Doerr RC,Wasserman AE. 1973. Use of sodium ascorbate or erythrobate to inhibit formation of N-nitroso-dimethylamine in frankfurters. J Food Sci 38: 1084-1091. 
    45. Choi HI, Ye EJ, Kim SJ, Bae MJ, Yee ST, Park EJ, ParkEM. 2006. Anticancer (in vitro) and antiallergy effects of rice bran extracts. J Korean Soc Food Sci Nutr 35: 1297-1303.     
    46. Bae MJ, Yee ST, Ye EJ. 2007. Anti-cancer and anti-allergy activities of mycelia extracts of Lentinus edodes mushroom-cultered Glycyrrhiza radix. J East Asian Soc DietaryLife 17: 43-50.     
    47. Bae MJ, Kim KJ, Kim SJ, Ye EJ. 2007. Effect of mycelia extracts from Lentinus edodes mushroom-cultered Astragalus membranaceus Bunge on anticancer and antiallergyactivities. J Korean Soc Food Sci Nutr 36: 8-13.     
    48. Bae MJ, Ye EJ. 2007. Effect of mycelia extracts from Lentinus edodes mushroom-cultered Lonicera japonica Thunberg on anticancer and antiallergy activities. J Korean Soc FoodSci Nutr 36: 424-430.     
    49. Choi JH, Yang HS, Shon MY, Kim JS, Park SK. 2004.Antioxidant and anticarcinogenic activities of methanol extracts from soybean products fermented by some mycelia of mushroom and Bacillus megaterium SMY-212. Food Industry and Nutrition 9: 34-39.     
  • 이 논문을 인용한 문헌 (11)

    1. Kim, Hoon ; Jeong, Jae-Hyun ; Shin, Ji-Young ; Kim, Dong-Goo ; Yu, Kwang-Won 2011. "Immunomodulatory and Anti-Inflammatory Activity of Mulberry (Morus alba) Leaves Fermented with Hericium erinaceum Mycelium by Solid-State Culture" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(9): 1333~1339     
    2. Lee, Sang-Il ; Lee, Ye-Kyung ; Choi, Jong-Keun ; Yang, Seung-Hwan ; Lee, In-Ae ; Suh, Joo-Won ; Kim, Soon-Dong 2012. "Quality Characteristics and Antioxidant Activity of Mulberry Leaf Tea Fermented by Monascus pilosus" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(5): 706~713     
    3. Seo, Hyeong-Ho ; Jeong, Jong-Moon 2013. "Beneficial Effects of Phyto-Extract Complex (CME) on Degenerative Arthritis" 大韓本草學會誌 = The Korea journal of herbology, 28(6): 87~93     
    4. Seo, Hyeong Ho ; Jeong, Jong Moon 2014. "Inhibitory Effects of Complex of Mulberry Extract on Degenerative Arthritis" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 22(4): 262~269     
    5. Jeong, Ji-Suk ; Kim, Yong-Joo ; Ahn, Eun-Kyung ; Shin, Ju-Young ; Go, Geun-Bae ; Son, Byeong-Gil 2015. "Antioxidative Activities and Qualitative Characteristics of Substitute Tea using Salvia plebeia R. Br." 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 31(1): 41~52     
    6. Kim, Jinku ; Ha, Jeongku ; Li, Hua ; Jeong, Jong-Moon 2015. "Animal and Clinical Study of the Efficacy of Mulberry Extract Complex on Degenerative Arthritis" 大韓本草學會誌 = The Korea journal of herbology, 30(3): 25~34     
    7. Jeon, Seo Young ; Kim, Ae Jeong ; Rho, Jeong Ok 2015. "A Study on the Physicochemical Activities of Dangmyon (starch vermicelli) Added with Mulberry Leaves Powder" 한국생활과학회지 = Korean journal of human ecology, 24(5): 713~723     
    8. Lee, Seon-Ho 2015. "Development of Mulberry-leaf Tea Containing γ-Aminobutyric Acid (GABA) by Anaerobic Treatments" 한국식품과학회지 = Korean journal of food science and technology, 47(5): 652~657     
    9. Cho, Myoung Lae ; Oh, Yu-Na ; Ma, Jin-Gyeong ; Lee, Su-Jin ; Choi, Young-Hee ; Son, Dong-Hwa ; Jang, Eun Hee ; Kim, Jong-Yea 2016. "Effect of extract temperature and duration on antioxidant activity and sensory characteristics of Ulmus pumila bark extract" 한국식품저장유통학회지 = Korean journal of food preservation, 23(7): 995~1003     
    10. Li, Hua ; Yun, Sat-Byul ; Shin, So Hee ; Jeong, Jong-Moon 2016. "Effect of Mulberry Extract Complex on Degenerative Arthritis In Vivo Models" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 45(5): 634~641     
    11. Choi, Kyung-Soon ; Kim, Yong-Hwan ; Shin, Kyung-Ok 2016. "Effect of Mulberry Extract on the Lipid Profile and Liver Function in Mice Fed a High Fat Diet" 한국식품영양학회지 = The Korean journal of food and nutrition, 29(3): 411~419     

 저자의 다른 논문

  • 배만종 (32)

    1. 1983 "폐기종실(廢棄種實)의 식량자원화(食糧資源化)에 관(關)하여 제(第) 4 보(報) : 고추씨의 화학적(化學的) 조성(組成)" 한국영양식량학회지 12 (1): 46~50    
    2. 1983 "폐기종실(廢棄種實)의 식량자원화(食糧資源化)에 관(關)하여 제(第)5보(報) : 수박씨의 화학적(化學的) 조성(組成)" 한국영양식량학회지 12 (3): 207~211    
    3. 1985 "한국인삼의 연근별 단백질 및 아미노산 조성" 한국식품과학회지 = Korean journal of food science and technology 17 (1): 1~4    
    4. 1985 "한국산 인삼 단백질의 용해성 및 전기영동 패턴" 한국농화학회지 = Journal of the Korean society of Agricultural Chemistry and Biotechnology 28 (2): 88~91    
    5. 1987 "냉이의 지방질(脂肪質) 및 지방산(脂肪酸) 조성에 관한 연구(硏究)" 한국영양식량학회지 16 (2): 83~86    
    6. 1990 "인삼 분획성분들이 고지방식이에 의해서 유도된 비만 Rat에서 혈장, 지방조직 및 변 Steroids에 미치는 영향" 고려인삼학회지 = Korean journal of ginseng science 14 (3): 404~415    
    7. 2004 "Arabinoxylane과 PSP의 면역세포 활성화 효과" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 33 (2): 278~286    
    8. 2004 "Arabinoxylane과 PSP의 항암 효과 및 알레르기 억제 효과 검증" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 33 (3): 469~474    
    9. 2004 "소건중탕 추출액과 노제의 세포증식 및 면역활성도 비교연구" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine 18 (1): 179~186    
    10. 2005 "전통적 탁주증자법으로 처리한 홍삼의 일부 항산화 및 항암효과" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 34 (5): 599~604    
  • 예은주 (13)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기