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Journal of food science and nutrition v.15 no.2, 2010년, pp.159 - 166  
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Development of Species-Specific Primers for PCR Identification of Lactobacillus hilgardii and Lactobacillus farciminis in Kimchi

Lee, Myung-Ki    (Korea Food Research Institute   ); Ku, Kyung-Hyung    (Korea Food Research Institute   ); Kim, Young-Jin    (Korea Food Research Institute   ); Kim, Kyung-Hee    (Korea Food Research Institute   ); Kim, Yu-Ri    (Korea Food Research Institute   ); Yang, Hye-Jung    (Korea Food Research Institute  );
  • 초록

    The aim of this study was to develop species-specific primer sets for kimchi Lactobacillus. Known gene sequences of Lactobacillus 16S rRNA were collected from the NCBI Gene bank, and 69 primer sets were designed using the homologous gene sequence. Six species of kimchi Lactobacilli were used as reference strains: Lactobacillus brevis KCTC3102, Lactobacillus farciminis KCTC3681, Lactobacillus fermentum KCTC3112, Lactobacillus hilgardii KCTC3500, Lactobacillus plantarum KCTC3099, and Lactobacillus sanfranciscensis KCTC3205. PCR amplification and gel electrophoresis were performed to identify the accuracy and specificity of the developed primer set. The results show that the primer set of 5'-aagcctgcgaaggcaag-3' & 5'-aggccaccggctttg-3', 5'-acatactatgcaaatctaagagattagacg-3' & 5'-actgagaatggctttaagagattagcttac-3' resulted in a specific PCR band on L. hilgardii, and primer set of 5'-ctaataccgcataacaactactttcacat-3' & 5'-aacttaataaaccgcctacattctctttac-3' on L. farciminis. The results indicate that the developed primer sets can provide a useful tool for the identification and differentiation of L. hilgardii and L. farciminis from other Lactobacillus species of kimchi.


  • 주제어

    Lactobacillus species .   Lb.   hilgardii .   Lb.   farciminis .   kimchi .   PCR primer.  

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