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Journal of microbiology and biotechnology v.20 no.3, 2010년, pp.569 - 573   SCIE
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Rapid Quantification of Salmonella in Seafood Using Real-Time PCR Assay

Kumar, Rakesh    (Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology   ); Surendran, P.K.    (Poothuvallil, Dr. Surendran Lane   ); Thampuran, Nirmala    (Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology  );
  • 초록

    A quantitative detection method for Salmonella in seafood was developed using a SYBR Green-based real-time PCR assay. The assay was developed using pure Salmonella DNA at different dilution levels [i.e., 1,000 to 2 genome equivalents (GE)]. The sensitivity of the real-time assay for Salmonella in seeded seafood samples was determined, and the minimum detection level was 20 CFU/g, whereas a detection level of 2 CFU/ml was obtained for pure culture in water with an efficiency of ${\geq}85%$ . The real-time assay was evaluated in repeated experiments with seeded seafood samples and the regression coefficient ( $R^2$ ) values were calculated. The performance of the real-time assay was further assessed with naturally contaminated seafood samples, where 4 out of 9 seafood samples tested positive for Salmonella and harbored cells


  • 주제어

    Salmonella .   seafood .   quantitative detection .   realtime PCR.  

  • 참고문헌 (24)

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