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한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology v.38 no.1, 2010년, pp.53 - 63   피인용횟수: 5
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

집먼지 진드기 항원으로 아토피 피부염을 유발한 NC/Nga 생쥐에 미치는 $\gamma$-PGA의 효과
The Effect of $\gamma$-PGA on NC/Nga Mice, a Mouse Model for Mite Antigen-induced Atopic Dermatitis

장순남    (건국대학교 대학원 생물공학과   ); 김금란    (건국대학교 대학원 생물공학과   ); 문미영    (대전대학교 뷰티건강관리학과   ); 강상모    (건국대학교 대학원 생물공학과  );
  • 초록

    $\gamma$ -PGA는 우리 전통 콩 발효식품인 청국장의 끈적끈적한 점액성 성분의 하나로, 매우 다양한 기능을 가지고 있는 천연물질이다. 이러한 $\gamma$ -PGA의 아토피발진 억제 효과를 알아보고자 NC/Nga 생쥐를 사용하여 in vivo 실험을 하였다. BMAC로 유도된 NC/Nga 아토피 피부발진 생쥐에 분자량 300 kDa인 $\gamma$ -PGA(PGA-HM)와 이를 저분자화 시킨 저분자 $\gamma$ -PGA(PGA-LM)을 경구 투여한 결과 PGA-LM 투여군에서 clinical skin severity score가 유의성 있게 감소하였다. 혈청 IgE 수준은 PGA-LM이 대조군에 비하여 유의적으로 감소하였고, 혈청 IgG1 수준은 대조군에 비하여 감소하였으나 두 군 모두 유의성이 없었다. $CD4^+CD25^+foxp3^+$ Treg 세포가 유도되는 것을 확인하기 위하여, PGA-HM와 PGA-LM를 투여한 NC/Nga 아토피 피부발진 생쥐의 등 부위에서 mRNA를 분리히여 real-time PCR로 foxp3 mRNA 유전자 발현량을 측정한 결과는 대조군에 비하여 PGA-LM 투여군이 약 2배 이상 증가를 나타내었다. 또한 등피부 조직의 조직검사에서도 epidermis의 두께, 비만세포 침윤, 그리고 $CCR3^+$ 세포수 등이 대조군에 비하여 현저히 억제됨을 관찰할 수 있었다. 이상의 결과로 PGA-HM보다 PGA-LM이 BMAC로 아토피 피부염이 유발된 NC/Nga 생쥐에 $CD4^+CD25^+foxp3^+$ Treg 세포를 활성화하여 IgE 및 염증 사이토카인의 생산 및 $CCR3^+$ 호산구의 활성화가 억제되어 아토피조절 효과를 나타내는 것으로 사료되었다.


    As one of the mucous components of Cheonggukjang, traditional fermented soybean paste, $\gamma$ -PGA is a natural substance with diverse functions. In this paper, an in-vivo experiment has been performed using NC/Nga mice in order to find out the efficacy of $\gamma$ -PGA in human atopic dermatitis. The NC/Nga mice with BMAC-induced atopic dermatitis were administered $\gamma$ -PGA (PGA-HM) with 300 kDa and low-molecular $\gamma$ -PGA (PGA-LM), respectively. As a result, a significant decrease in clinical skin severity score was detected in the group that was administered PGA-LM. In terms of serum IgE levels, a significant decline was observed in PGA-LM, compared to the control group. The serum IgG1 levels also decreased more in PGA-LM than in the control group. However, no significant difference was observed in both groups. To witness the induction of $CD4^+CD25^+foxp3^+$ Treg cells, mRNA was sampled from the back of PGA-HM- and PGA-LM-administered NC/Nga mice with atopic dermatitis. In terms of the production amount of foxp3 mRNA, which was measured in real-time PCR, the group that was administered PGA-LM was twice as high as the control group. According to a biopsy on the skin on the backs of the mice, the experimental group was also far lower than the control group in terms of epidermis thickness, mast cell infiltration and the number of $CCR3^+$ cells. Therefore, it has been confirmed that the atopic dermatitis symptoms decreased more in the PGA-LM-administered NC/Nga mice than the PGA-HM-administered group by facilitating $CD4^+CD25^+foxp3^+$ Treg cells and suppressing the activity of eosinophils and production of IgE and pro-inflammatory cytokines.


  • 주제어

    $\gamma$-PGA .   $CD4^+CD25^+foxp3^+$ (Treg) .   BMAC .   NC/Nga.  

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  • 이 논문을 인용한 문헌 (5)

    1. Kim, Min-A ; Son, Hyeong-U ; Nam, Dong-Yoon ; Cha, Yong-Su ; Shin, Yong-Kyu ; Choi, Yong-Hee ; Lee, Sang-Han 2012. "Inhibitory Effect of Angelica keiskei Extract in an Atopic Dermatitis Animal Model" 한국식품저장유통학회지 = Korean journal of food preservation, 19(5): 792~798     
    2. 2012. "" Food science and biotechnology, 21(2): 475~483     
    3. Kim, Bae Jin ; Son, Woo Rim ; Choi, Mi Ok ; Jo, Seung Kyeung ; Jung, Hee Kyoung ; Lee, Jin Tae ; Kim, Hak Yoon ; Kwoen, Dae Jun 2013. "Anti-atopic Effects of Castanea crenata Inner Shell Extracts Fermented by Lactobacillus bifermentans" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(9): 1378~1386     
    4. Lee, Moon Hee ; Han, Min Ho ; Yoon, Jung Jeh ; Song, Myung Kyu ; Kim, Min Ju ; Hong, Su Hyun ; Choi, Byung Tae ; Kim, Byung Woo ; Hwang, Hye Jin ; Choi, Yung Hyun 2014. "Medicinal Herb Extracts Attenuate 1-Chloro-2,4dinitrobenzene-induced Development of Atopic Dermatitis-like Skin Lesions" 생명과학회지 = Journal of life science, 24(8): 851~859     
    5. Kim, Yun Hee ; Lee, Hye Lim 2015. "Therapeutic Effects of Sesamum Indicum Extracts on Atopic Dermatitis-Like Skin Lesions of NC/Nga Mouce" 大韓韓方小兒科學會誌 = The journal of pediatrics of Korean medicine, 29(4): 39~66     

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