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한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology v.38 no.1, 2010년, pp.70 - 76   피인용횟수: 1
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장환형 단일가닥 DNA를 이용한 암세포 성장 억제 유전자 발굴
Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes

배윤위    (영남대학교 의과대학 생리학교실   ); 문익재    (웰진   ); 서영배    (경북대학교 생명공학부   ); 도경오    (영남대학교 의과대학 생리학교실  );
  • 초록

    The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.


  • 주제어

    Microarray .   expression profiling .   microarray .   antisense .   lung cancer.  

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    1. Ryu, Ki-Jun ; Cho, Sung-Hee 2011. "Effects of Angelicae Gigantis Radix on Gene Expression of Ovarian Tissue in Polycystic Ovary Syndrome Rats" 大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, 24(3): 28~47     

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