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한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology v.38 no.1, 2010년, pp.77 - 83   피인용횟수: 12
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

항생제 다제내성 Pseudomonas aeruginosa 및 Candida 균주에 대한 산사자의 항균 활성
Antimicrobial Activity of Fruit of Crataegus pinnatifida Bunge against Multidrug Resistant Pathogenic Pseudomonas aeruginosa and Candida sp.

류희영    (안동대학교 식품영양학과   ); 안선미    (안동대학교 식품영양학과   ); 김종식    (안동대학교 생명과학과   ); 정인창    (안동대학교 식품영양학과   ); 손호용    (안동대학교 식품영양학과  );
  • 초록

    산사자는 전 세계적으로 이용되는 있는 식용/약용 생물자원 중 하나이다. 본 연구에서는 산사자의 유용 생리활성 검토를 위한 연구의 일환으로, 산사자의 methanol 추출물 및 이의 n-hexane, ethylacetate, butanol 분획물 및 물 잔류물을 조제하여 항생제 다제내성 Pseudomonas aeruginosa 및 Candida sp.를 포함하는 다양한 병원성 및 식중독 미생물에 대한 항균활성을 평가하였다. 산사자의 methan이 추출물은 그람 양성 및 음성의 다양한 세균에 대해 항균활성을 나타내였고, 이의 분획물 중 ethylacetate 및 butanol 분획물은 Listeria monocytogen, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Proteus vulgaris, Escherichia coli는 물론 10종의 항생제 내성 병원성 Pseudomonas aeruginosa에 대해서도 우수한 항세균 활성을 나타내었다(최소생육억제농도 1.0~7.5 mg/mL). 또한 ethylacetate 및 butan이 분획물은 일부의 Candida sp.에 대해서도 항균활성을 나타내였다. 한편 n-hexane 분획물을 제외한 산사자 methan이 추출물 및 분획물들은 $500\;{\mu}g/mL$ 농도까지 인간적혈구에 대한 용혈현상을 보이지 않았으며, n-hexane 분획물은 $500\;{\mu}g/mL$ 농도에서 약 9.9%의 미미한 용혈활성을 나타내었다. 이러한 결과는 산사자가 다양한 세균의 제어는 물론 항생제 내성 Pseudomonas aeruginosa 제어를 위한 생물자원으로 개발 기능함을 제시하고 있다.


    The fructus of Crataegus pinnatifida Bunge (CBF) has been used as medicinal and food source in worldwide. In this study, antimicrobial activity of the methanol extract and its sequential organic solvent fractions of CBF against different pathogenic bacteria and fungi, including multidrug resistant Pseudomonas aeruginosa and Candida sp., were investigated. The methanol extract of CBF was active against various gram-positive and gram-negative bacteria, and the ethylacetate and butanol fractions of CBF showed strong antibacterial activity against Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Proteus vulgaris, Escherichia coli and various multidrug resistant Pseudomonas aeruginosa with minimal inhibitory concentration of 1.0~7.5 mg/mL. Also the fractions showed anti-Candida activity against C. albicans, C. kruseis and C. geochares. The methanol extract of CBF and its solvent fractions, except n-hexane fraction, did not show any hemolytic activity against human red blood cell up to $500\;{\mu}g/mL$ , respectively. The hemolysis in n-hexane fraction at $500\;{\mu}g/mL$ was less than 9.9%. Our results suggest that the CBF could be developed as a potent antibacterial agent, especially for multidrug resistant Pseudomonas aeruginosa.


  • 주제어

    Antibacterial .   multidrug resistant Pseudomonas aeruginosa .   anti-Candida .   fructus of Crataegus pinnatifida Bunge.  

  • 참고문헌 (30)

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  • 이 논문을 인용한 문헌 (12)

    1. Yu, Young-Eun ; Park, Eun-Young ; Jung, Dae-Hwa ; Byun, Sung-Hui ; Kim, Sang-Chan ; Park, Sung-Min 2010. "Antibacterial Activity of Oriental Medicinal Herb Extracts against Skin Pathogens" 생명과학회지 = Journal of life science, 20(7): 1143~1150     
    2. Ahn, Seon-Mi ; Choi, Tae-Ho ; Kwun, In-Sook ; Sohn, Ho-Yong 2011. "Antifungal Activity of Methylene Chloride Fraction of Pimpinella brachycarpa Against Aspergillus niger" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 39(2): 168~174     
    3. Yu, Young-Eun ; Kim, Ok-Ah ; Kim, Sang-Chan ; Park, Sung-Min 2011. "The Antibacterial Effects of Oriental Medicinal Herbs on Bacteria Isolated from Contaminated Beverages" Korean journal of microbiology = 미생물학회지, 47(3): 241~248     
    4. Ahn, Seon-Mi ; Kim, Mi-Sun ; Jung, In-Chang ; Sohn, Ho-Yong 2011. "Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa" 한국식품저장유통학회지 = Korean journal of food preservation, 18(4): 590~596     
    5. Kim, Mi-Sun ; Kwon, Kyung-Jin ; Lee, Min-Jin ; Ahn, Seon-Mi ; Sohn, Ho-Yong 2012. "Evaluation of the Antimicrobial Activities of 35 Seaweed Extracts against Pathogenic Bacteria and Candida sp." 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 40(2): 144~151     
    6. Kim, Min-A ; Duan, Yishan ; Seong, Jong-Hwan ; Chung, Hun-Sik ; Kim, Han-Soo 2014. "Antioxidative Activity of Feral Haw (Crataegus pinnatifida BUNGE) Seed Extracts Using Various Solvents" 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 30(1): 33~40     
    7. Seong, Jong-Hwan ; Kim, Min-A ; Duan, Yishan ; Kim, Han-Soo ; Lee, Young-Guen ; Kim, Dong-Seob ; Chung, Hun-Sik 2014. "Effects of Feral Haw (Crataegus pinnatifida Bunge) on Hepatic Functional Enzyme Activity in Dyslipidemic Rats" 東아시아食生活學會誌 = Journal of the East Asian Society of Dietary Life, 24(2): 176~182     
    8. Kim, Han-Soo ; Kim, Min-A ; Jang, Seong-Ho 2014. "Improvement Effect of Hyperlipidemia by Wild Haw (Crataegus pinnatifida BUNGE)" Journal of environmental science international = 한국환경과학회지, 23(5): 787~792     
    9. Kim, Han-Soo ; Kim, Min-A ; Jang, Seong-Ho 2014. "Influences of Korean Haw (Crataegus pinnatifida BUNGE) on Lipid Concentration in Hypercholesterolemia" Journal of environmental science international = 한국환경과학회지, 23(5): 793~800     
    10. Kim, Han-Soo ; Kim, Min-A ; Duan, Yishan ; Jang, Seong-Ho ; Cho, Han-Jin ; Ryu, Jae-Young ; Kim, Sang-Woo 2014. "Influences of Wild Haw (Crataegus pinnatifida BUNGE) on Lowering BUN and Creatinine Concentrations in Dyslipidemia" Journal of environmental science international = 한국환경과학회지, 23(6): 1029~1035     
    11. Duan, Yishan ; Kim, Min-A ; Seong, Jong-Hwan ; Lee, Young-Guen ; Kim, Dong-Seob ; Chung, Hun-Sik ; Kim, Han-Soo 2014. "Impacts of Various Solvent Extracts from Wild Haw(Crataegus pinnatifida Bunge) Pulpy on the Antioxidative Activities" 東아시아食生活學會誌 = Journal of the East Asian Society of Dietary Life, 24(3): 392~399     
    12. Kim, Han-Soo ; Duan, Yishan ; Kim, Min-A ; Jang, Seong-Ho 2014. "Contents of Antioxidative Components from Pulpy and Seed in Wild Haw (Crataegus pinnatifida BUNGE)" Journal of environmental science international = 한국환경과학회지, 23(11): 1791~1799     

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