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한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology v.38 no.1, 2010년, pp.105 - 111   피인용횟수: 5
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

소목 추출물의 항균활성과 Brazilin의 구조분석
Antibacterial Activities of Caesalpinia sappan L. Extract and Structural Analysis of Its Related Brazilin

권현정    (순천향대학교 생명과학과   ); 김용현    (순천향대학교 생명과학과   ); 남궁우    (정산생명공학(주)   ); 김선기    (선 바이오(주)   ); 방인석    (호서대학교 생명과학과   ); 한만덕    (순천향대학교 생명과학과  );
  • 초록

    소목은 전통적으로 타박상, 염증 등의 질병치료를 위해 민간의학에서 오래 동안 사용되어 온 한약재야다. 소목의 섬재로부터 항균효과를 조사하기 위하여 MeOH 추출에 의한 hexane, $CHCl_3$ , EtOAc, n-BuOH, MeOH 및 $H_2O$ 분획을 얻었다. 분획 시료 중 3.94%의 가장 높은 수율의 EtOAc 분획이 조사된 인채 병원성 세균에 대해 가장 강한 항균활성 나타냈다. 또한 EtOAc 분획의 항균활성은 Gram (-) 균주 보다 Gram (+) 균주에 더 효과적이며, Gram (-) 균주에 대해서는 세균에 따른 항균활성의 차이를 보였다. EtOAc 분획은 silica gel adsorption column chromatography 및 Sephadex LH-20을 이용하여 분리하였으며 수득한 주요 성분은 채결정을 통하여 얻어진 단일물질을 HPLC를 이용하여 순도를 측정하였다. 300g의 C. sappan으로부터 3.94%의 EtOAc 분획을 얻었으며 EtOAc분획물은 분석한 결과 10 mg에는 1.67 mg의 brazilin을 포함하였다. 분리된 활성울질은 짧은 황색 결정의 단일 화합물로 MS에 의해 분자량이 285로 나타났으며, $^1H$ -NMR, $^{13}C$ -NMR에 의한 구조분석으로 brazilin( $C_{16}H_{14}O_5$ 을 동정하였다. 이 같은 결과는 소목의 MeOH 추출에 의한 EtOAc 분획에 존재하는 brazilin이 S. aureus와 같은 인체 병원성 Gram (+) 균주에 대한 생약치료제로 활용이 가능할 것을 시사한다.


    Caesalpinia sappan L. has long been commonly used in oriental folk medicines to treat diseases. To investigate the antibacterial effects from C. sappan L. heart wood, the MeOH soluble extract was successively fractionated by using hexane, $CHC1_3$ , EtOAc, BuOH, MeOH, and $H_2O$ . Among of these extracts, the EtOAc fraction which partitioned to 3.94% of the highest yields was to be the most active against all human pathogenic bacteria in this experiment. In addition, the antibacterial activities of the EtOAc fraction were more effective against Gram (+) bacteria compared to those against Gram (-) bacteria, which showed difference of the antibacterial activities against Gram (-) bacteria. To confirm the identity of the active substances, the EtOAc fraction was further separated by silica gel adsorption column, high performance liquid chromatography, and 98.48% purity of brazilin (1.67 mg)/EtOAc (10 mg) fraction was obtained from 300 g of C. sappan L. heart wood. The isolated active substance was a single compound of yellow crystalline, and was identified as brazilin ( $C_{16}H_{14}O_5$ ) by MS, and $^lH$ -NMR and $^{13}C$ -NMR. These results suggest that the brazilin in the EtOAc fraction from MeOH extract of C. sappan L. has a potential as a natural therapeutic agent against human pathogenic Gram (+) bacteria such as Staphylococcus aureus.


  • 주제어

    Caesalpinia sappan L.   .   ethyl acetate fraction .   antibacterial activity .   brazilin .   structural analysis.  

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    1. Jeon, Mi-Ae ; Kwon, Hyun-Jung ; Kim, Yong-Hyun ; Han, Kook-Il ; Chung, Ha-Na ; Chung, Sung-Kyun ; Bang, In Seok ; Han, Man-Deuk 2010. "Antioxidative Activities and Antiproliferation Effects on Oral Carcinoma KB Cell of the Brazilin from Caesalpinia sappan L." 치위생과학회지= Journal of dental hygiene science, 10(5): 365~372     
    2. Yu, Young-Eun ; Park, Eun-Young ; Jung, Dae-Hwa ; Byun, Sung-Hui ; Kim, Sang-Chan ; Park, Sung-Min 2010. "Antibacterial Activity of Oriental Medicinal Herb Extracts against Skin Pathogens" 생명과학회지 = Journal of life science, 20(7): 1143~1150     
    3. Lee, Jin-Young ; Min, Kyung-Jin 2011. "Antimicrobial Activity and Bactericidal Activity of Caesalpinia sappan L. Extract" 韓國環境保健學會誌 = Journal of environmental health sciences, 37(2): 133~140     
    4. Eum, Won Sik ; Lee, Kwang-Jae ; Kim, Dae Won ; Lim, Soon Sung ; Kang, Il-Jun ; Park, Jinseu ; Choi, Soo Young 2013. "Anti-Inflammatory Effects of Extracts from Caesalpinia sappan L. on Skin Inflammation" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(3): 384~388     
    5. Kim, Yi-Seul ; Shim, Hye-Mee ; Kim, Kwang-Yup 2016. "Antimicrobial Effect of Caesalpinia sappan L. Extract on Foodborne Bacteria" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 45(7): 1026~1034     

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