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KSBB Journal v.25 no.1, 2010년, pp.85 - 90  

이온선택성 미소전극을 이용한 질산화 생물막내의 질소 이온의 농도 모니터링
Monitoring of Nitrogen Ion in Nitrifying Biofilm using an Ion Selective Microelectrode

선지윤    (부산대학교 사회환경시스템공학과   ); 변임규    (부산대학교 환경기술산업개발연구센터   ); 이태호    (부산대학교 사회환경시스템공학과   ); 박태주    (부산대학교 사회환경시스템공학과  );
  • 초록

    An ion selective microelectrode (ISME) was fabricated to measure concentrations of ammonium ( ${NH_4}^+$ -N) and nitrate ( ${NO_3}^-$ -N) according to the depth of nitrifying biofilm. The limits of detectability and validity of results were investigated to evaluate the ISME. The electromotive force (EMF) was directly proportional to the ion concentration, and average detection limits of ${NH_4}^+$ and ${NO_3}^-$ ISME were $10^{-5.14}$ and $10^{-5.18}$ M, respectively. The concentrations of ${NH_4}^+ $ -N and ${NO_3}^-$ -N in various depths on the nitrifying biofilm were measured by the ISME. When a modified Ludzack-Ettinger (MLE) process was operated at an HRT of 6 h, concentration gradients of ${NH_4}^+$ -N in the bulk solution and biofilm at depths of $100\;{\mu}m$ decreased by $70\;{\mu}M$ , while ${NO_3}^-$ -N increased by $101\;{\mu}M$ and remained constant thereafter. At an HRT of 4 h, concentration gradients of ${NH_4}^+$ -N at depths of $500\;{\mu}m$ decreased by $160\;{\mu}M$ and ${NO_3}^-$ -N increased by $162;{\mu}M$ and remained constant thereafter. As HRT decreased, the concentration gradients of ${NH_4}^+$ -N and ${NO_3}^-$ -N between bulk solution and biofilm was higher due to the increase of nitrogen load. Also, the concentration gradients of the ${NH_4}^+$ -N and ${NO_3}^-$ -N of biofilm in the second aerobic tank were lower than those of the first aerobic tank, suggesting differences of nitrogen load and concentrations of DO between the first and second aerobic tank.


  • 주제어

    ion selective microelectrode .   biofilm .   ion profile .   $NH_4{^+}$-N .   $NO_3{^-}$.  

  • 참고문헌 (17)

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    8. Kim, Y. O., T. J. Park, and T. H. Lee (2003) Analysis fo morphology, microbial activity and distribution in aerophilic biofilms by in situ hybridization methods, J. of KSEE. 25: 803-809. 
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    16. Matsumoto, S., A. Terada, and S. Tsuneda (2007) Modeling of membrane-aerated biofilm: effects of C/N ratio, biofilm thickness and surface loading of oxygen on feasibility of simultaneous nitrification and denitrification. J. Biochem. Eng. 37: 98-107. 
    17. Liu, Y. and B. Capdeville (1996) Specific activity of nitrifying biofilm in water nitrification process. Wat. Res. 30: 1645-1650. 

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