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Biotechnology and bioprocess engineering v.15 no.3, 2010년, pp.400 - 406   SCIE 피인용횟수: 2
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Antioxidant Activity of the Chemical Constituents from the Flower Buds of Magnolia denudata

Seo, Young-Wan    (Division of Marine Environment and Bioscience, Korea Maritime University  );
  • 초록

    The scavenging activity of the flower buds of Magnolia denudata Desrousseaux on reactive oxygen species (ROS) was evaluated using 2',7'-dichlorofluorescin diacetate (DCFH-DA) in HT 1080 cells. Methanol (MeOH) and dichloromethane ( $CH_2Cl_2$ ) extracts inhibited dosedependently generation of ROS in the cellular system. MeOH and $CH_2Cl_2$ extracts were combined and fractionated with n-hexane, 85% aqueous MeOH, and n-butanol (n-BuOH). Both n-hexane-soluble and 85% aqueous-soluble fractions showing strong radical-scavenging activity in the cellular system were further separated by diverse chromatographic methods to give five known lignans (1-5). All these compounds exhibited significant radical-scavenging effect on intracellular ROS in a dose-dependent manner. Their scavenging activity on various reactive oxygen species (ROS) was also evaluated using electron spin resonance (ESR) spin-trap techniques.


  • 주제어

    Magnolia denudata .   antioxidant activity .   reactive oxygen species .   ESR.  

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  • 이 논문을 인용한 문헌 (2)

    1. Lee, Jung-Im ; Kong, Chang-Suk ; Jung, Myoung-Eun ; Hong, Joo-Wan ; Lim, Sun-Young ; Seo, Young-Wan 2011. "Antioxidant Activity of the Halophyte Limonium tetragonum and Its Major Active Components" Biotechnology and bioprocess engineering, 16(5): 992~999     
    2. Mok, So-Youn ; Shin, Hyun-Cheol ; Lee, Sang-Hyun 2012. "Screening of aldose reductase inhibitory activity of white-color natural products" 농업과학연구 = CNU Journal of agricultural science, 39(1): 69~73     

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