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Biotechnology and bioprocess engineering v.15 no.3, 2010년, pp.453 - 459   SCIE
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Effects of Ascorbic Acid and Uracil on Exo-polysaccharide Production with Hericium erinaceus in Liquid Culture

Lee, Jong-Seok    (Department of Bioengineering and Technology, Kangwon National University   ); Wee, Jae-Wan    (Department of Bioengineering and Technology, Kangwon National University   ); Lee, Hye-Young    (Department of Bioengineering and Technology, Kangwon National University   ); An, Hyo-Sil    (Department of Bioengineering and Technology, Kangwon National University   ); Hong, Eock-Kee    (Department of Bioengineering and Technology, Kangwon National University  );
  • 초록

    Hericium erinaceus is a well known edible and medicinal mushroom used in East-Asia. Recently, H. erinaceus has attracted a lot of attention owing to its anti-tumor, immuno-modulatory, and cytotoxic effect. It has been postulated that the fruiting body of H. erinaceus contains a polysaccharide that is similar to ${\beta}$ -D-glucan, which is known to have antitumor activity against Sarcoma 180. However, optimized liquid culture conditions for enhanced polysaccharide productivity have yet to be developed, which is a necessary step for industrial applications. Therefore, the aim of this study was to determine the optimal liquid culture conditions for maximum polysaccharide production. In shake flask cultures, the optimal concentration of ascorbic acid was found to be 2.0 g/L, which prevented the broth from changing color from yellow to black. The optimal culture conditions were determined to be $23^{\circ}C$ , 200 rpm, and a 10% inoculum size, at an uncontrolled initial pH. In addition, the modified medium contained 20 g/L glucose, 10 g/L yeast extract, and 2.0 g/L ascorbic acid. The maximum mycelial biomass and exo-polysaccharide (EPS) production in the modified medium containing uracil was 13.43 and 1.26 g/L, respectively.


  • 주제어

    Hericium erinaceus .   exo-polysaccharide .   ascorbic acid .   uracil .   mycelial morphology.  

  • 참고문헌 (32)

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