본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Biotechnology and bioprocess engineering v.15 no.3, 2010년, pp.481 - 487   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Microwave-assisted Extraction of Homoharringtonine from Cephalotaxus koreana

Kim, Won-Kyo    (Department of Chemical Engineering, Kongju National University   ); Chae, Hee-Jeong    (Department of Food and Biotechnology, Hoseo University   ); Kim, Jin-Hyun    (Department of Chemical Engineering, Kongju National University  );
  • 초록

    An efficient method using microwave energy was developed to extract homoharringtonine (HHT), an alkaloid component effective in the treatment of leukemia, from Cephalotaxus koreana. The effects of major process parameters on extraction efficiency were also investigated. Using a fixed biomass-to-methanol ratio of 1:8 (w/v), an extraction temperature of $30^{\circ}C$ , an extraction time of 20 min, and a stirrer velocity of 250 rpm, a 25% higher yield of HHT was achieved using microwave-assisted extraction (MAE) than using conventional solvent extraction. It was possible to recover more than 95% of the HHT by extracting twice using MAE. In addition, the HHT yield increased as the extraction temperature increased, but the content of plant-derived tar and waxy compounds increased as well. Removal of these impurities and of the pigments from extracts was most effectively accomplished at a mixing ratio of biomass-to-sylopute of 1:1.5 (w/w). The effects of using different organic solvents (acetone, chloroform, ethanol, or methanol) for MAE were also assessed; the highest extraction efficiency was obtained using methanol. When the agitation speed was altered, most of the HHT (> 99%) was recovered at 250 rpm. A mixing ratio of biomass-to-methanol of 1:6 (w/v) at an extraction temperature of $40^{\circ}C$ and an extraction time of 10 min proved to be the most effective for reducing processing time and organic solvent usage while enabling nearly all of the HHT (> 99%) to be recovered.


  • 주제어

    Cephalotaxus koreana .   homoharringtonine(HHT) .   microwave-assisted extraction (MAE) .   optimization of extraction conditions.  

  • 참고문헌 (33)

    1. Powell, R. G., D. Weisleder, and C. R. Smith (2000) Antitumor alkaloids from Cephalotaxus harringtonia: Structure and activity. J. Pharm. Sci. 61: 1227-1230. 
    2. Kantarjian, H. M., M. Talpaz, V. Santini, A. Murgo, B. Cheson, and S. M. O'Brien (2001) Homoharringtonine: History, current research, and future directions. Cancer 92: 1591-1605. 
    3. Ohnuma, T. and J. F. Holland (1985) Homoharringtonine as a new antileukemic agent. J. Clin. Oncol. 3: 604-606. 
    4. Tuebaeva, R. M., D. M. Graifer, N. B. Matasova, O. S. Fedorova, V. B. Odintsov, N. A. Aitkhozhina, and G. G. Karpova (1992) Selective inhibition of the polypeptide chain elongation in eukaryotic cells. Biochim. Biophys. Acta. 1129: 177-182. 
    5. Visani, G., D. Russo, E. Ottaviani, P. Tosi, D. Damiani, A. Michelutti, S. Manfroi, M. Baccarani, and S. Tura (1997) Effects of homoharringtonine alone and in combination with alpha interferon and cytosine arabinoside on 'in vitro' growth and induction of apoptosis in chronic myeloid leukemia and normal hematopoietic progenitors. Leukemia 11: 624-628. 
    6. He, J., A. P. Cheung, E. Wang, E. Struble, K. Fang, N. Nguyen, and P. Liu (2008) Stability-indicating LC assay of and impurity identification in homoharringtonine samples. J. Pharm. Biomed. Anal. 22: 541-554. 
    7. Zhang, W. G., F. X. Wang, and Y. X. Chen (2008) Combination chemotherapy with low-dose cytarabine, homoharringtonine, and granulocyte colony-stimulating factor priming in patients with relapsed or refractory acute myeloid leukemia. Am. J. Hematol. 83: 185-188. 
    8. Quintas-Cardama, A. and H. Kantarjian (2007) Phase I/II study of subcutaneous homoharringtonine in patients with chronic myeloid leukemia who have failed prior therapy. Cancer 109: 248-255. 
    9. Stone, R. M., K. A. Donohue, and W. Stock (2009) A phase II studies of continuous infusion homoharringtonine and cytarabine in newly diagnosed patients with chronic myeloid leukemia: CALGB study 19804. Cancer Chemother. Pharmacol. 63: 859-864. 
    10. Bell, B. A., M. N. Chang, and H. J. Weinstein (2001) A phase II study of homoharringtonine for the treatment of children with refractory or recurrent acute myelogenous leukemia: A pediatric oncology group study. Med. Pediatr. Oncol. 37: 103-107. 
    11. Kim, B. S. and J. H. Kim (2009) Characterization of solvent induced crystalline and amorphous homoharringtonine. Kor. J. Chem. Eng. 26: 1090-1093. 
    12. Keller, L., F. Dumas, and J. d'Angelo (2001) Enantioselective synthesis of the ester side chain of homoharringtonine. Tetrahed. Lett. 42: 1911-1913. 
    13. Liu, J., H. Yan, and K. H. Row (2009) Simultaneous quantification of multiple alkaloids in Sophora flavescens Ait and human urine by HPLC. Biotechnol. Bioproc. Eng. 14: 675-679.     
    14. Jeon, K. Y. and J. H. Kim (2007) Optimization of micellar extraction for the pre-purification of paclitaxel from Taxus chinensis. Biotechnol. Bioproc. Eng. 12: 354-358.     
    15. Hyun, J. E. and J. H. Kim (2008) Microwave-assisted extraction of paclitaxel from plant cell cultures. Kor. J. Biotechnol. Bioeng. 23: 281-284.     
    16. Jeong, H. J., S. A. Kim, J. H. Kwon, and H. K. Kim (2008) Physiological activities of Gardeniae Fructus extracts by microwave- assisted extraction as affected by solvents. J. Kor. Soc. Food Sci. Nutr. 37: 282-287.     
    17. Kwon, J. H., Y. H. Choi, H. W. Chung, and G. D. Lee (2005) The characteristics of a microwave extraction process used for saikosaponins from Bupleurum falcatum root. Intern. J. Food Sci. Technol. 41: 67-75. 
    18. Zheng, X., X. Wang, Y. Lan, J. Shi, S. J. Xue, and C. Liu (2009) Application of response surface methodology to optimize microwave-assisted extraction of silymarin from milk thistle seeds. Sep. Purif. Technol. 70: 34-40. 
    19. Marie, E. L., S. Jacqueline, B. Steven, L. Willem, and C. Farid (2007) Solvent free microwave extraction of Elletaria cardamomum L.: A multivariate study of a new technique for the extraction of essential oil. J. Food Eng. 79: 1079-1086. 
    20. Mandal, V., Y. Mohan, and S. Hemalatha (2007) Microwave assisted extraction-an innovative and promising extraction tool for medicinal plant research. Pharmacog. Rev. 1: 7-18. 
    21. Sung, J. L., B. S. Kim, and J. H. Kim (2005) Development of a pre-purification process for homoharringtonine. J. Chem. Technol. Biotechnol. 80: 1148-1153. 
    22. Thostenson, E. T. and T. W. Chou (1999) Microwave processing: Fundamentals and applications. Composites Part A-Appl. Sci. Manuf. 30: 1055-1071. 
    23. Zhang, B., R. Yang, and C. Z. Liu (2008) Microwave-assisted extraction of chlorogenic acid from flower buds of Lonicera japonica Thunb. Sep. Purif. Technol. 62: 480-483. 
    24. Pan, X., H. Liu, G. Jia, and Y. Y. Shu (2000) Microwaveassisted extraction of glycyrrhizic acid from licorice root. Biochem. Eng. J. 5: 173-177. 
    25. Camel, V. (2000) Microwave-assisted solvent extraction of environmental samples. Trends Anal. Chem. 19: 229-248. 
    26. Xiao, W., L. Han, and B. Shi (2008) Microwave-assisted extraction of flavonoids from Radix astragali. Sep. Purif. Technol. 62: 614-618. 
    27. Kim, B. S. and J. H. Kim (2008) Purification of homoharringtonine and removal of residual solvents by spray drying. Kor. J. Chem. Eng. 25: 108-111. 
    28. Pyo, S. H., H. B. Park, B. K. Song, B. H. Han, and J. H. Kim (2004) A large-scale purification of paclitaxel from cell cultures of Taxus chinensis. Proc. Biochem. 39: 1985-1991. 
    29. Pyo, S. H., B. K. Song, C. H. Ju, B. H. Han, and H. J. Choi (2005) Effects of absorbent treatment on the purification of paclitaxel from cell cultures of Taxus chinensis and yew tree. Proc. Biochem. 40: 1113-1117. 
    30. Kovacs, A., K. Ganzler, and L. Simon-Sarkadi (1998) Microwave-assisted extraction of free amino acids from foods. Z. Lebensm. Unters. Forsch. A. 207: 26-30. 
    31. Eskilsson, C. S. and E. Bjorklund (2000) Analytical-scale microwave-assisted extraction. J. Chromatogr. A. 902: 227-250. 
    32. Chen, Y., M. Y. Xie, and X. F. Gong (2007) Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. J. Food Eng. 81: 162-170. 
    33. Youn, Y. S., Y. K. Ming, and S. C. Yuan (2003) Microwaveassisted extraction of ginsenosides from ginseng root. Microchem. J. 74: 131-139. 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기