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생명과학회지 = Journal of life science v.20 no.2 = no.118, 2010년, pp.237 - 244   피인용횟수: 6

유용곰팡이 균주에 의한 발효 누에분말의 이화학적 특성 및 생리활성
Biological Activity and Chemical Characteristics of Fermented Silkworm Powder by Mold

차재영    (대선주조(주) 기술연구소   ); 김용순    (국립농업과학원 농업생물부   ); 강필돈    (국립농업과학원 농업생물부   ); 안희영    (동아대학교 생명공학과   ); 엄경은    (동아대학교 생명공학과   ); 조영수    (동아대학교 생명공학과  );
  • 초록

    미생물 발효에 의한 누에분말의 생리활성 작용 및 물질을 개발하기 위한 기초 자료를 얻을 목적으로 Mucor racemosus forma racemosus KCCM 12352, Rhizopus oryzae KCCM 11273, A. oryzae KCCM 11372, A. kawachii KCCM 32819 및 Monascus purpureus KCCM 12002 5종의 곰팡이 균주를 본 실험에 사용하였으며, 이들에 의한 발효 누에분말의 미네랄 함량, 지방산 조성, 단백질 함량, 단백질 패턴, 항산화 활성 및 혈전용해 활성을 비교 검토하였다. 발효 동결 및 열풍건조 누에분말의 주요 미네랄 함량은 각각 칼륨 72.0~76.3 및 77.1~78.9 ppm, 마그네슘 29.6~49.7 및 44.3~58.7 ppm, 칼슘 1.9~14.9 및 9.8~21.6 ppm 및 아연 0.64~0.70 및 4.17~4.52 ppm이였으며, 주요지방산 조성은 linolenic acid, oleic acid 및 palmitic acid 이였다. 발효 동결 및 열풍건조 누에 분말의 단백질을 Native-PAGE로 비교분석 한 결과 발효 동결누에 분말에서 많은 종류의 밴드가 관찰되었으며, SDS-PAGE상의 단백질 패턴 분석에서는 97~66 kDa 중간 정도 크기의 단백질 밴드가 발효에 의해 분해되어 거의 관찰되지 않았다. 발효 동결 및 열풍건조 발효누에 분말의 항산화 활성은 발효전보다 증가하였으나 균주 종류별로는 큰 차이가 없었고, 시판 항산화제 BHT 처리구의 90% 이상의 높은 항산화 활성보다는 낮았다. 혈전용해 활성은 동결 및 열풍건조 누에분말 모두 없었으나, 열풍건조 누에분말의 A. kawachii 및 Monascus purpureus에 의한 발효누에에서 혈전용해 활성이 나타났다. 이상의 실험결과에서 곰팡이 균주에 의한 누에분말의 생리활성 효과 및 이화학적 특성이 발효전 누에 분말보다 강화됨으로써 건강기능식품의 소재로서의 이용 가능성이 높다고 할 수 있다.


    Five mold strains - Mucor racemousus, Rhizopus oryzae, Aspergillus oryzae, Aspergillus kawachii, and Monascus purpureus - were used for the fermentation of both freeze-dried and air-dried silkworm powders. The concentrations of proteins and minerals, electrophoretical protein patterns, fatty acid composition and the activities of fibrinolytic and antioxidation with freeze-dried silkworm (FDSW) or air-dried silkworm powders (ADSW) fermented by the five molds were investigated. The concentrations of major minerals in fermented FDSW and ADSW powders were K by 72.0-76.3 and 77.1-78.9 ppm, Mg by 29.6-49.7 and 44.3-58.7 ppm, Ca by 1.9-14.9 and 9.8-21.6 ppm and Zn by 0.64-0.70 and 4.17-4.52 ppm, respectively. Major fatty acids in fermented FDSW and ADSW powders were linolenic acid, oleic acid, and palmitic acid. There were slightly varietal differences in electrophoretical protein patterns when total protein patterns of fermented FDSW and ADSW powders were analyzed by native-and SDS-polyacrylamide gel electrophoresis (PAGE). DPPH radical scavenging activity was slightly stronger in fermented ADSW powders than that in fermented FDSW. Fibriolytic activity was only detected in the FDSW fermented by Aspergillus kawachi and Monascus purpureus. These results may provide the basic data to understand the biological activities and chemical characteristics of silkworm fermented by mold, which can be used for the development of functional foods.


  • 주제어

    Fermented-silkworm powder .   mold strains .   antioxidant activity .   fibriolytic activity .   functional foods.  

  • 참고문헌 (45)

    1. AOAC. 1997. Official methods of analysis. 17th eds., Association of official analytical chemists. Washington, DC, USA. 
    2. Arena, M. E. and M. C. Manca de Nadra. 2005. Influence of ethanol and low pH on arginine and citrulline metabolism in lactic acid bacteria from wine. Res. Microbiol. 156, 858-864. 
    3. Astrup, T. and S. Mullertz. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351. 
    4. Bliznakov, E. G. 2000. More on the Chinese red-yeast-rice supplement and its cholesterol-lowering effect. Am. J. Clin. Nutr. 71, 152-154. 
    5. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204. 
    6. Cha, J. Y., H. Y. Jung, J. J. Jeong, H. J. Yang, Y. T. Kim, and Y. S. Lee. 2009. Effects of amino acids on the activities of alcohol metabolizing enzyme alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). J. Life Sci. 19, 1321-1327.     
    7. Cha, J. Y., Y. Mameda, K. Oogami, K. Yamamoto, and T. Yanagita. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohydrolase activity in rats. Biosci. Biotechnol. Biochem. 62, 508-513. 
    8. Cha, J. Y., Y. S. Kim, H. Y. Ahn, K. E. Eom, B. K. Park, and Y. S. Cho. 2009. Biological activity of fermented silkworm powder. J. Life Sci. 19, 1468-1477.     
    9. Cha, J. Y., J. C. Park, H. Y. Ahn, K. E. Eom, B. K. Park, B. S. Jun, and Y. S. Cho. 2009. Effect of Monascus purpureus fermented Korean red ginseng powder on the serum lipid levels and antioxidative activity in rats. J. Korea Soc. Food Sci. Nutr. 38, 1153-1160.     
    10. Cho, C. H., W. S. Cha, and J. S. Kim. 1989. Effect of temperature, time and pH on the extraction of protein in a chrysalis of silkworm. Korean J. Biotechnol. Bioeng. 4, 65-68. 
    11. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42. 
    12. Davis, B. J. 1964. Disc electrophoresis-II. Method and application to human serum proteins. In gel electrophoresis. by J. E. Fredrich. Ann. New York Acad. Sci. 121-404. 
    13. Endo, A. 1979. Monacolin-K, a new hypocholesterolemic agent produced by Monascus species. J. Antibiot. 32, 852-854. 
    14. Garces, R. and M. Mancha. 1993. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal. Biochem. 211, 139-143. 
    15. Gu, Y. A., S. Y. Jang, N. Y. Park, C. R. Mun, O. M. Kim, and J. Y. Jeong. 2006. Property changes of mung bean depending on hydrolysis conditions. Korean J. Food Presrev. 13, 563-568.     
    16. Huang, C. F., T. C. Li, C. C. Lin, C. S. Liu, H. C. Shih, and M. M. Lai. 2007. Efficacy of Monascus purpureus Went rice on lowering lipid ratios in hypercholesterolemic patients. Eur. J. Cardiovasc. Prev. Rehabil. 4, 438-440. 
    17. Ikeda, I., J. Y. Cha, T. Yanagita, N. Nakatani, K. Oogamil, K. Imaizumi, and K. Yazawa. 1998. Effects of dietary $\alpha$-linolenic, eicosapentaenoic and docosahexaenoic acids on hepatic lipogenesis and $\beta$-oxidation in rats. Biosci. Biotechnol. Biochem. 62, 675-680. 
    18. Jeon, C. P., C. S. Kim, J. B. Lee, J. W. Shin, S. Y. Choi, C. S. Choi, O. S. Lee, and G. S. Kwon. 2007. Characteristics of Monascus natural pigments producd by Monascus sp. MK2-2. J. Life Sci. 17, 137-142.     
    19. Kang, G. D., K. H. Lee, S. G. Do, C. S. Kim, J. G. Suh, Y. S. Oh, and J. H. Nham. 2001. Effect of silk fibroin on the protection of alcoholic hepatotoxicity in the liver of alcohol preference mouse. Int. J. Indust. Entomol. 2, 15-18. 
    20. Kang, K. S., H. Y. Kim, J. S. Pyo, and T. Yokozawa. 2003. Increase in the free radical scavenging activity of ginseng by heat-processing. Biol. Pharm. Bull. 29, 750-754. 
    21. Kang, P. D., H. J. Yoon, K. S. Ryu, B. H. Sohn, and H. D. Sohn. 1999. Electrophoretic patterns of hemolymph proteins of varieties with long and short life span in the silkworm Bombyx mori L. Korean J. Seric. Sci. 41, 1-8. 
    22. Kato, N., S. Sato, A. Yamanaka, H. Yamada, N. Fuwa, and M. Nomura. 1998. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci. Biotechnol. Biochem. 62, 145-147. 
    23. Kim, I. J., H. K. Kim, J. H. Jong, Y. K. Jeong, and C. H. Ryu. 2002. Study of functional Chungkukjang contain fibrinolytic enzyme. J. Life Sci. 12, 357-362.     
    24. Kim, K. T., K. M. Yoo, J. W. Lee, S. H. Eom, I. K. Hwang, and C. Y. Lee. 2007. Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharmacol. 111, 443-450. 
    25. Kim, Y. S., K. Y. Kim, P. D. Kang, J. Y. Cha, J. S. Heo, and Y. S. Cho. 2008. Effect of silkworm (Bombyx mori) excrement powder on the alcoholic hepatotoxicity in rats. J. Life Sci. 18, 1342-1347.     
    26. Lee, J. H., N. K. Kim, D. Y. Lee, and C. H. Lee. 1999. Protective effect of selected amino acids and food extracts on ethanol toxicity determent in rat liver. Korean J. Food Sci. Technol. 31, 802-808. 
    27. Lee, S., W. G. Kim, E. Kim, I. J. Ryoo, H. K. Lee, J. N. Kim, S. H. Jung, and I. D. Yoo. 2005. Synthesis and melanin biosynthesis inhibitory activity of (+/-)-terrein produced by Penicillium sp. 20135. Bioorg. Med. Chem. Lett. 15, 471-473. 
    28. Nicoli, M. C., M. Anese, L. Manzocco, and C. R. Lerici. 1997. Antioxidant properties of coffee brews in relation to the roasting degree. Food Sci. Technol. 30, 292-297. 
    29. Lee, T. S. and J. Y. Choi. 2005. Volatile flavor components in mash of Takju prepared by using Aspergillus kawachii nuruks. Korean J. Food Sci. Technol. 37, 944-950.     
    30. Ma, J., Y. Li, Q. Ye, J. Li, Y. Hua, D. Ju, D. Zhang, R. Cooper, and M. Chang. 2000. Constituents of red yeast rice, a traditional Chinese food and medicine. J. Agric. Food Chem. 48, 5220-5225. 
    31. Ok, M., W. S. Seo, J. Y. Cha, and Y. S. Cho. 2001. Isolation and characterization of Bacillus sp. WRD-2 extracellular protease from soil. J. Korean Soc. Agric. Chem. Biotechnol. 44, 246-250. 
    32. Ok, M. and Y. S. Cho. 2005. Screening of fibrinolytic enzyme producing from microorganism in Korean fermented soybean paste and optimum conditions of enzyme production. Korean J. Food Preserv. 12, 643-649.     
    33. Park, C. D., H. J. Jung, H. W. Lee, H. S. Kim, and T. S. Yu. 2005. Antioxidant activity of Monascus pigment of Monascus purpureus P-57 mutant. Korean J. Microbiol. 41, 135-139.     
    34. Park, Y. J., J. C. Heo, S. M. An, E. Y. Yun, S. M. Han, J. S. Hwang, S. W. Kang, C. Y. Yun, and S. H. Lee. 2005. High throughput-compatible screening of antioxidative substances by insect extract library. Korean J. Food Preserv. 12, 482-488.     
    35. Patrick, L. and M. Uzick. 2001. Cardiovascular disease: C-reactive protein and the inflammatory disease paradigm: HMG-CoA reductase inhibitors, alpha-tocopherol, red yeast rice, and olive oil polyphenols. A review of the literature. Altern. Med. Rev. 6, 248-271. 
    36. Pyo, Y. H. and T. C. Lee. 2007. The potential antioxidant capacity and angiotensin I-converting enzyme inhibitory activity of Monascus-fermented soybean extracts: evaluation of Monascus-fermented soybean extracts as multifunctional food additives. J. Food Sci. 72, S218-223. 
    37. Rhyu, M. R. and E. Y. Kim. 2002. The relations between antihypertensive effect and $\gamma$-aminobutyric acid, mycelial weight and pigment of Monascus. Korean J. Food Sci. Technol. 34, 737-740.     
    38. Ryu, H. Y., J. C. Heo, J. S. Hwang, S. W. Kang, C. Y. Yun, S. H. Lee, and H. Y. Sohn. 2008. Screening of thrombin inhibitor and its DPPH radical scavenging activity from wide insects. J. Life Sci. 18, 363-368.     
    39. Seong, S. I., K. E. Park, M. Nagata, and N. Yoshitake. 1985. Effect of metamorphois on the major hemolymph proteins of the silkworm. Arch. Insect Biochem. Physiol. 2, 91-104. 
    40. Sumi, H., H. Hamada, H. Tsushima, H. Mihara, and H. Muraki. 1978. A novel fibrinolytic enzyme (Nattokinase) in the vegetable che in Natto: a typical and popular soybean food in the Japanese diet. Experimentia 43, 1110-1111. 
    41. Suzuki, Y., K. J. Choi, K. Uchida, S. R. Ko, H. J. Sohn, and J. D. Park. 2004. Arginyl-fructosyl-glucose and arginyl-fructose, compounds related to browning reaction in the model system of steaming and heat-drying processes for the preparation of red ginseng. J. Ginseng Res. 28, 143-148.     
    42. Tsukahara, M., N. Shinzato, Y. Tamaki, T. Namihira, and T. Matsui. 2009. Red yeast rice fermentation by selected Monascus sp. with deep-red color, lovastatin production but no citrinin, and effect of temperature-shift cultivation on lovastatin production. Appl. Biochem. Biotechnol. 158, 476-482. 
    43. Weber, K. and M. Osborn. 1969. The reliability of molecular weight determination by sodium dodesyl sulphate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406-4412. 
    44. Yin, M., K. Ikejima, G. E. Arteel, V. Seabra, B. U. Bradford, H. Kono, I. Rusyn, and R. G. Thurman. 1998. Glycine accelerates recovery from alcohol-induced liver injury. J. Pharacol. Exp. Ther. 286, 1014-1019. 
    45. Yun, E. Y., S. H. Kim, W. S. Kang, B. R. Jin, K. Y. Kim, H. R. Kim, M. S. Han, and S. K. Kang. 1997. Molecular cloning and expression of the novel attacin-like antibacterial protein gene isolated from the Bombyx mori. Korean J. Appl. Entomol. 36, 331-340. 
  • 이 논문을 인용한 문헌 (6)

    1. Cha, Jae-Young ; Kim, Yong-Soon ; Ahn, Hee-Young ; Eom, Kyung-Eun ; Heo, Su-Jin ; Cho, Young-Su 2010. "Bioactive and Chemical Properties by Silkworm (Bombyx mori L.) Powder Degradation with Kiwifruit, Papaya, Pineapple and Pear Juice" 생명과학회지 = Journal of life science, 20(11): 1718~1724     
    2. Cha, Jae-Young ; Kim, Hyun-Woo ; Heo, Jin-Sun ; Ahn, Hee-Young ; Eom, Kyung-Eun ; Heo, Su-Jin ; Cho, Young-Su 2010. "Ingredients Analysis and Biological Activity of Fermented Angelica gigas Nakai by Mold" 생명과학회지 = Journal of life science, 20(9): 1385~1393     
    3. Cha, Jae-Young ; Kim, Yong-Soon ; Ahn, Hee-Young ; Kang, Min-Jung ; Heo, Su-Jin ; Cho, Young-Su 2011. "Biological Activity and Biochemical Properties of Silkworm (Bombyx mori L.) Powder Fermented with Bacillus subtilis and Aspergillus kawachii" 생명과학회지 = Journal of life science, 21(1): 81~88     
    4. Ahn, Hee-Young ; Cha, Jae-Young ; Cho, Young-Su 2012. "Biological Activity and Chemical Characteristics of Fermented Acanthopanax senticosus by Mold" 생명과학회지 = Journal of life science, 22(12): 1704~1711     
    5. Ahn, Hee-Young ; Cha, Jae-Young ; Park, Kyu-Rim ; Kim, Yu-Ra ; Cho, Young-Su 2013. "Improvement Effect of Fermented Silkworm (Bombyx mori L.) Powder against Orotic Acid-induced Fatty Liver in Rats" 생명과학회지 = Journal of life science, 23(6): 789~795     
    6. Hwang, Seong-Hee ; Jang, Jae-Seon ; Kim, Min-Jeong ; Kim, Kang-Sung 2014. "The Change of Free Sugar, Hesperidine, Naringin, Flavonoid Contents and Antihypertensive Activities of Yuza Variety according to Harvest Date" 한국식품영양학회지 = The Korean journal of food and nutrition, 27(6): 1051~1058     

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