본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

韓國營養學會誌 = The Korean journal of nutrition. v.43 no.1, 2010년, pp.12 - 25   피인용횟수: 3

뼈건강 지표를 이용한 유기태 칼슘 급원의 생체이용성 및 소화율
Bioavailability and Digestibility of Organic Calcium Sources by Bone Health Index

한정호    (고려대학교 생명공학원   ); 김은미    (한국식품연구원   ); 정만기    (고려대학교 생명공학원   ); 지성규    ((주)삼풍B&F   ); 지규만    (고려대학교 생명공학원  );
  • 초록

    본 연구는 $CaCO_3$ 를 표준으로 장내 칼슘 흡수를 촉진하는 peptide-Ca, anchovy-Ca, methionine hydroxyl analogue calcium의 생체이용성과 소화율을 평가하였다. 표 준군은 $CaCO_3$ 를 AIN-93G (1993)를 기준으로 칼슘 요구량의 0%, 30%, 60%로 첨가하여 모든 분석 항목에 대한 비교기준으로 삼았다. 실험군은 요구량의 40% 수준으로 각기 다른 칼슘 공급원으로 실험하였다. 실험결과는 대부분 칼슘 함량이 높은 Ca-60%군이 가장 높은 수치를 보여 주어 칼슘의 섭취량과 비례하여 양의 상관 관계를 보여 주었다. 생체이용성 평가에서는 MHA-Ca 군이 체중 증가량, BMD, BS, BBS 항목에서 높은 이용성을 보여 주었다. peptide-Ca은 회귀계수가 높은 회분함량 ( $R^2\;=\;0.98$ )과 BMC ( $R^2\;=\;0.935$ )를 이용한 평가 에서 표준군에 비해 11%, 7% 높은 이용성을 보여주었다. Anchovy-Ca군 역시 여러 생체이용성 평가항목에서 높은 이용성을 나타냈다. 이는 동일한 양의 칼슘을 섭취했을 때 실험군이 표준으로 사용된 $CaCO_3$ 보다 높은 칼슘 이용성을 가지고 있음을 의미한다. 하지만 본 연구로는 유기태 칼슘이 어떤생리 활성 작용을 통해 이러한 결과를 나타냈는지는 알 수 없었으며, 이 부분을 분명하게 규명하는 추가적인 연구가 필요한 것으로 판단된다. 소화율 측정항목에서는 Ca-0%군을 제외하고는 90% 이상 높은 칼슘 소화율을 나타냈다. 소화율과 보유율에서 peptide-Ca군이 유의한 차이는 없었지만 다른 실험군에 비해 미세하게 높았다. 이러한 차이의 축적으로 인해 생체이용성 평가시 실험군간 차이를 보여 주었다. 결국 공급한 칼슘에 대한 소화 및 흡수의 차이에 의해 뼈 건강관련 지표가 좋아지고 이로 인해 실험군의 생체 이용성이 높게 평가 되었다. Peptide-Ca와 순수한 식품 칼슘 급원인 멸치 뼈는 높은 칼슘 이용성을 나타내어, 체내칼슘 영양개선에 상당한 효과가 있슴이 증명되었다.


    This study was carried out to evaluate the bioavailabilities and the digestibilities of oligopeptide chelated (peptide-Ca), anchovy bone (anchovy-Ca) and methionine hydroxyl analogue (MHA-Ca) calcium compared to those of calcium carbonate in rats. In exp1, $CaCO_3$ , were added to the basal diet at level of 0, 30 and 60% calcium of the AIN-93G diet. In test groups, peptide-Ca, anchovy-Ca and MHA-Ca, were added to the basal diet to provide calcium at the level of 40% of AIN-93G. In exp1, the bioavailabilities were evaluated from the regression equation of the ratios of theological/ actual calcium intakes of each dietary treatment. In exp2, urine and feces was to evaluate the true- and apparent digestibility and apparent retention. In exp1, Ca-60% group had higher bone mineral density (BMD), bone mineral content (BMC) and bone breaking strength (BBS) than those of the other standard groups. The bone weight and ash content of the peptide-Ca and anchovy-Ca groups were significantly higher than those of the MHA-Ca. Bone calcium content were not significantly different from the test group. The bioavailability of the MHA-Ca group was shown higher BMD (71%), BS (38%) and BBS (27%) compared to another control group. But the regression coefficient for BMD, BS and BBS were lower compare with that of bone ash and BMC. In exp2, the true- and apparent digestibility of test groups were shown to over 90%. Peptide-Ca was not significantly different from other test group, but digestibility and retention were higher compare to other test groups. In conclusion, peptide-Ca, anchovy-Ca and MHA-Ca improved Ca bioavailability in the rats. The compounds were higher Ca digestibility compared with those of $CaCO_3$ . It is assumed that difference of digestibility for test groups may be correlated to the bioavailability of test groups in BMD, BMC, BS, BBS and bone ash respectively.


  • 주제어

    calcium .   bioavailability .   digestibility .   bone calcium .   rat.  

  • 참고문헌 (43)

    1. Korea Centers for Disease Control and Prevention, The third Korea National Health and Nutrition Examination Survey (KNHANES III); 2005 
    2. Allen LH. Calcium bioavailability and absorption: a review. Am J Clin Nutr 1982; 35: 783-808 
    3. Heribert J, Watzke A. Impact of processing on bioavailability examples of minerals in foods. Trends in Food Sci & Technol 1998; 9: 320-327 
    4. Susan J, Fairweather-Tait SJ. Iron and Calcium Bioavaila-bility. In: Forti editor. Foods and Dietary Supplements. New York. International Life Sciences Institute; 2002. p.360-367 
    5. Morohashi T, Sano T, Ohta A, Yamada S. True calcium absorption in intestine is enhanced by fructooligosaccharide feeding in rats. J Nutr 1998; 128: 1815-1818 
    6. Rath NC, Huff WE, Balog JM and Bayyari GR. Effect of gonadal steroids on bone and other physiological parameters of male broiler chickens. Poul Sci 1996; 75: 556-562 
    7. Kwon OR, Kim MK. Effects of dietary Ca levels and kinds of lipid on the lipid metabolism in the rats. Korean J Nutr 1988; 21(5): 324-332 
    8. Mehta S. Bone elasticity and ultrasound velocity are affected by subtle change in organic matrix. J Bone Miner Res 1998; 13: 114-119 
    9. Sammom PE. Role of parathyroid hormone in calcium homeostasis and metabolism. Am J Physiol 1970; 218: 479-485 
    10. Sakhaee K, Bhuket T, Adams-Huet B, Rao DS. A meta-analysis of calcium bioavailability: a comparison of calcium citrate with calcium carbonate. Am J Therapeutics 1999; 6: 313-321 
    11. Duan Y, Parfitt AM, Seeman E. Vertebral bone mass, size, and volumetric density in women with spinal fractures. J Bone Miner Res 1999; 14: 1796-1802 
    12. Korean Pharmaceutical Association. Production sheet of medicines pharmaceutics in Korea. Seoul; 1995 
    13. Cordey J, Schneider M, Belendez C, Ziegler WJ, Rahn BA, Perren SM. Effect of bone size, not density, on the stiffness of the proximal part of normal and osteoporotic human femora. J Bone Miner Res 1992; 7(Suppl2): S437-S444 
    14. Yendt ER, Cohanim M, Jarzylo S. Reduced glomerular filtration and a renal tubular Ca leak in womae with primary osteoporosis. J Bone Min Res 1989; 4(s): 253-256 
    15. Ezawa I. Studies on calcium metabolism. Jap J Home Econo 1987; 38: 695-703 
    16. Turner RT. Basic Biomechanical Measurements of Bone. A Tutorial Bone 1993; 14: 595-608 
    17. Vega E, Ghiringhelli G, Mautalen C, Rey VG, Scaglia H, Zylberstein C. Bone mineral density and bone size in men with primary osteoporosis and vertebral fractures. Calcif Tissue Int 1998; 62: 465-469 
    18. Coe FL, Parks JH. Recurrent renal calcium. Cause and Prevention. Hosp Prac 1986; 30: 49-57 
    19. Iacono JM. Effect of varying dietary level of calcium on plasma and tissue lipids of rabbits. J Nutr 1974; 104: 1165-1171 
    20. Lee JH, Moon SJ, Huh KB. Influence of phytate and low dietary calcium on calcium, phosphate and zinc metabolism by growing rats. Korean J Nutr 1993; 26(2): 145-155 
    21. Smith EL, Gilligan C, Smith PE, Sempos CT. Calcium supplementation and bone loss in middle-aged woman. Am J Clin Nutr 1989; 50: 833-842 
    22. Sheikh MS, Anta ACA, Nicar MJ. Gastrointestinal absorptions of calcium from milk and calcium salt. N Engl J med 1987; 317: 532-536 
    23. Robert PH. Factors Influencing the Measurement of Bioavailability, Taking Calcium as a Model1. Am Society for Nutritional Sciences; 2001 
    24. Chung HK, Chang N, Lee HS, Chang YE. The effect of various types of calcium sources on calcium and bone metabolism in rats. Korean J Nutr 1996; 29(5): 480-488 
    25. Sobal J, Muncie HL. Vitamin/mineral supplements use among adolescent. J Nutr Edu 1988; 20(6): 314-318 
    26. Levenson DI, Bockman RS. A review of calcium preparations. Nutr Rev 1994; 52: 221-232 
    27. Meulen MCH. Understanding bone strength: size isn't everything. Bone 2001; 29: 101-104 
    28. Roland DA. Calcium metabolism in the laying hen: The calcium status of the hen at night. Poult Sci 1973; 52: 351-354 
    29. Linder MC. Nutrition and metabolism of major minerals. In: Linder MC, editor. Nutritional biochemistry and metabolism with clinical application. New York; Elsevier; 1991. p.191-214 
    30. Yacowitz H. Effects of dietary calcium upon lipid metabolism in rats fed saturated or unsaturated fat. J Nutr 1967; 92: 389-392 
    31. Rath NC. Factors regulating bone maturity and strength in poultry. Poultry Sci 1999; 79: 1024-1032 
    32. Benson JD. Effect of previous calcium intake on adaptation to low and high calcium diets in rats. J Nutr 1969; 97: 53-60 
    33. Matkovic V. Factors that influence peak bone mass formation: A study of calcium balance and the inheritance of bone mass in adolescent females. Am J Clin Nutr 1990; 52: 878-888 
    34. Gueguen L, Pointillart A. The bioavailability of dietary calcium. J Am Coll Nutr 2000; 2: 119S-136S 
    35. The Korean Nutrition Society, Dietary Reference Intakes for Koreans, Seoul; 2005 
    36. Allen LH, Wood RJ. Calcium and phosphorus. In: Shils ME, Olson. JA M. eds.: Modern Nutrition in Health and Disease, 8th Ed; 1994. p.144-163 
    37. Fairweather-Tait SJ, Teucher B. Calcium bioavailability in relation to bone health. Int J Vitam Nutr Res 2001; 72: 13-18 
    38. Harms RH, Russell GB. Adding methinionine and lysine to broiler breeder diets to lower feed costs. J A Poul Res 1998; 7(2): 202-218 
    39. Le'on Gue'guen, MsScAgr. The Bioavailability of Dietary Calcium. J Am Coll Nutr 2000; 19(2): 119S-136S 
    40. Lewis AJ. Bioavailability of D-amino acids and DL-Hydroxymethionine. In: Ammerman CB, Baker DH and Lewis AJ, editor. Bioavailability of Nutrients for Animals. San Diego: Academic Press; 1995. p.67-81 
    41. Foley MK. Influence of dietary calcium and cholecalciferol on composition of plasma lipids in young pigs. J Nutr 1990; 120: 45-51 
    42. Roberfroid MB, Cumps J, Devogelaer JP. Dietary chicory inulin increases whole body bone mineral density in growing male rats. J Nutr 2002; 132: 3599-3602 
    43. Lee SH, Hwangbo YS, Kim JY, Lee YS. A study on the bioavailability of dietary calcium sources. Korean J Nutr 1997; 30(5): 499-505 
  • 이 논문을 인용한 문헌 (3)

    1. Lee, Ji-Hye ; Jeon, So-Jeong ; Song, Kyung-Bin 2010. "Isolation of a Calcium-Binding Fraction from a Hot-Water Extract of Smilax rhizoma" 한국식품저장유통학회지 = Korean journal of food preservation, 17(6): 903~907     
    2. Yoon, Gun-Ae ; Kim, Kwang-Hyeon 2010. "Effect of Calcium Source using Tilapia Mossambica Scales on the Bone Metabolic Biomarkers and Bone Mineral Density in Rats" 韓國營養學會誌 = The Korean journal of nutrition., 43(4): 351~356     
    3. Kim, Hyang Suk ; Choi, Eun Ok ; Kim, Man Do ; Choi, Yung Hyun ; Kim, Byung Woo ; Kim, Soo Yeon ; Hwang, Hye Jin 2013. "Effect of Calcium Extracted from Salted Anchovy (Engraulis japonicus) on Calcium Metabolism of the Rat" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(2): 182~187     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 한국영양학회 : 저널
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기