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Biomolecules & therapeutics v.18 no.3, 2010년, pp.262 - 270   SCIE SCOPUS 피인용횟수: 1
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Roles of Neutral Sphingomyelinase 1 on CD95-Mediated Apoptosis in Human Jurkat T Lymphocytes

Lee, Hyun-Min    (College of Pharmacy, Chung-Ang University   ); Surh, Bo-Young    (College of Pharmacy, Chung-Ang University   ); Chun, Young-Jin    (College of Pharmacy, Chung-Ang University  );
  • 초록

    CD95 receptor is a member of tumor necrosis factor receptor family that mediates apoptosis in many cell types when bound by CD95 ligand or cross-linked by agonistic anti-CD95 antibodies. To determine the role of neutral sphingomyelinase (nSMase) on CD95-mediatd apoptosis, human Jurkat T lymphocytes were exposed to recombinant human CD95 ligand. Treatment with CD95 ligand induced cell death in a concentration and time-dependent manner. CD95-induced cell death was suppressed by inhibitors of SMase such as AY9944 or desipramine. Transfection with human nSMase1 siRNA plasmid into CD95 ligand-treated cells significantly prevented CD95-mediated cell death. CD95-mediated elevation of intracellular ceramide level detected by FACS analysis with anti-ceramide antibody was also decreased by nSMase1 siRNA. Knock-down of nSMase1 expression also blocked cytochrome c release into cytosol and caspase-3 cleavage in CD95-treated cells. Taken together, these results suggest that nSMase1 may play an important role in CD95-mediated apoptotic cell death in Jurkat T cells.

  • 주제어

    CD95 ligand .   nSMase1 .   Jurkat T cell .   siRNA .   Apoptosis.  

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