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BMB reports v.43 no.6, 2010년, pp.432 - 437   SCIE 피인용횟수: 1
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Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

Deng, Yun    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University   ); Li, Yongqing    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University   ); Fan, Xiongwei    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University   ); Yuan, Wuzhou    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University   ); Xie, Huaping    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University   ); Mo, Xiaoyang    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University   ); Yan, Yan    (The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life  ); Zhou, Junmei   Wang, Yuequn   Ye, Xianli   Wan, Yongqi   Wu, Xiushan  
  • 초록

    LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes ${\alpha}B$ -crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with ${\alpha}B$ -crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, ${\alpha}B$ -crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or ${\alpha}B$ -crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both ${\alpha}B$ -crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and ${\alpha}B$ -crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.


  • 주제어

    LBH .   p21 .   p53 .   Yeast two-hybrid .   ${\alpha}B$-crystallin.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2012. "" BMB reports, 45(10): 577~582     

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