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BMB reports v.43 no.6, 2010년, pp.438 - 444   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Zebrafish Dnd protein binds to 3'UTR of geminin mRNA and regulates its expression

Chen, Shu    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Zeng, Mei    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Sun, Huaqin    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Deng, Wenqian    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Lu, Yilu    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Tao, Dachang    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Liu, Yunqiang    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Zhang, Sizhong    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University   ); Ma, Yongxin    (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University  );
  • 초록

    Dnd (dead end) gene encodes an RNA binding protein and is specifically expressed in primordial germ cells (PGCs) as a vertebrate-specific component of the germ plasma throughout embryogenesis. By utilizing a technique of specific nucleic acids associated with proteins (SNAAP), 13 potential target mRNAs of zebrafish Dnd (ZDnd) protein were identified from 8-cell embryo, and 8 target mRNAs have been confirmed using an RT-PCR analysis. Of the target mRNAs, the present study is focused on the regulation of geminin, which is an inhibitor of DNA replication. Using electrophoretic mobility shift assay (EMSA), we demonstrated that ZDND protein bound the 67-nucleotide region from 864 to 931 in the 3'UTR of geminin mRNA, a sequence containing 60.29% of uridine. Results from a dual-luciferase assay in HEK293 cells showed that ZDND increases the translation of geminin. Taken together, the identification of target mRNA for ZDnd will be helpful to further explore the biological function of Dnd in zebrafish germ-line development as well as in cancer cells.


  • 주제어

    Dead end .   Geminin .   RNA-binding protein .   Zebrafish.  

  • 참고문헌 (22)

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