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Nutrition research and practice v.4 no.3, 2010년, pp.183 - 190   SCIE 피인용횟수: 1
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Protective effect of enzymatic hydrolysates from highbush blueberry (Vaccinium corymbosum L.) against hydrogen peroxide-induced oxidative damage in Chinese hamster lung fibroblast cell line

Senevirathne, Mahinda    (School of Food Technology and Nutrition, Chonnam National University   ); Kim, Soo-Hyun    (Department of Food Bioengineering, Jeju National University   ); Jeon, You-Jin    (School of Marine Biomedical Science, Jeju National University  );
  • 초록

    Blueberry was enzymatically hydrolyzed using selected commercial food grade carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) and proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex) to obtain water soluble compounds, and their protective effect was investigated against $H_2O_2$ -induced damage in Chinese hamster lung fibroblast cell line (V79-4) via various published methods. Both AMG and Alcalase hydrolysates showed higher total phenolic content as well as higher cell viability and ROS scavenging activities, and hence, selected for further antioxidant assays. Both AMG and Alcalase hydrolysates also showed higher protective effects against lipid peroxidation, DNA damage and apoptotic body formation in a dose-dependent fashion. Thus, the results indicated that water soluble compounds obtained by enzymatic hydrolysis of blueberry possess good antioxidant activity against $H_2O_2$ -induced cell damage in vitro.

  • 주제어

    Blueberry .   enzymatic hydrolysates .   water soluble compounds .   $H_2O_2$ induced damage .   ROS.  

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  • 이 논문을 인용한 문헌 (1)

    1. Cho, Byoung Ok ; Lee, Chang-Wook ; So, Yangkang ; Jin, Chang-Hyun ; Yook, Hong-Sun ; Byun, Myung-Woo ; Jeong, Yong-Wook ; Park, Jong Chun ; Jeong, Il-Yun 2014. "Protective Effect of Radiation-induced New Blackberry Mutant γ-B201 on H2O2-induced Oxidative Damage in HepG2 Cells" 한국식품과학회지 = Korean journal of food science and technology, 46(3): 384~389     

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