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Food science and biotechnology v.19 no.3, 2010년, pp.683 - 689   SCIE
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A Simple Colorimetric Determination of the Free Amino Groups in Water Soluble Chitin Derivatives Using Genipin

Ramos-Ponce, Luz M.    (Research and Development Center, Coyotefoods Biopolymer and Biotechnology Co., Unidad de Biotecnologia, Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco (CIATEJ)   ); Vega, Mireille    (Research and Development Center, Coyotefoods Biopolymer and Biotechnology Co.   ); Sandoval-Fabian, Georgina C.    (Unidad de Biotecnologia, Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco (CIATEJ)   ); Colunga-Urbina, Edith    (School of Chemistry, Universidad Autonoma de Coahuila   ); Balagurusamy, Nagamani    (School of Biological Science, Universidad Autonoma de Coahuila   ); Rodriguez-Gonzalez, Francisco J.    (Plastic Processing Department, Centro de Investigacion en Quimica Aplicada (CIQA)   ); Contreras-Esquivel, Juan Carlos    (School of Chemistry, Universidad Autonoma de Coahuila  );
  • 초록

    A colorimetric method for quantitative measurement of free amino groups of water soluble chitin derivatives is described. The method utilizes genipin as a natural and specific reagent for determining the concentration of free amino groups in samples of water soluble chitin derivatives. The blue color adduct (complex) formed during genipin reaction with free amino groups was measured at about 589 nm and Beer-Lambert's law obeyed over the concentration range of 50 to 300 mg/L. Parameters of analytical conditions were considered and kept constant during the experimental procedure. Highly acetylated water soluble chitin derivatives can be differentiated from water soluble chitosan using this genipin method. The colorimetric method with genipin was proved to be a rapid and efficient technique to determine the free amino groups in water soluble chitin derivatives. This method can also be applied for the detection of the enzymatic activity of chitindeacetylase.


  • 주제어

    Genipa americana .   Gardenia jasmonides .   glucosamine .   chitin .   chitosan .   oligosaccharide.  

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