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Food science and biotechnology v.19 no.3, 2010년, pp.697 - 702   SCIE 피인용횟수: 2
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Purification and Characterization of a Novel Fibrinolytic Enzyme from Chive (Allium tuberosum)

Chung, Dong-Min    (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology, Department of Microbiology, Chungnam National University   ); Choi, Nack-Shick    (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology   ); Maeng, Pil-Jae    (Department of Microbiology, Chungnam National University   ); Chun, Hyo-Kon    (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology   ); Kim, Seung-Ho    (Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology  );
  • 초록

    A novel Allium tuberosum fibrinolytic enzyme (ATFE) was purified from the leaf of chive by ion exchange chromatography followed by gel filtration. The molecular mass and iso-electric point (pI) of ATFE were 90 kDa and 4.0 by using 1- or 2-D fibrin zymography, respectively. ATFE was optimally active at pH 4.0 and $40^{\circ}C$ . ATFE had a high degrading activity for the $A{\alpha}$ -chain of human fibrinogen and hydrolyzed the $B{\beta}$ -chain slowly, but did not affect the $\gamma$ -chain, indicating that it is a $\alpha$ -fibrinogenase. The proteolytic activity of ATFE was inhibited completely by phenylmethylsulfonyl fluoride (PMSF), indicating that this enzyme belongs to the serine protease class. ATFE was also inhibited by the 1 mM of $Cu^{2+}$ . ATFE exhibited high specificity for Meo-Suc-Arg-Pro-Tyr-pNA (S-2586), a synthetic chromogenic substrate for chymotrypsin. The first 20 amino acid residues of the N-terminal sequence of ATFE were determined as TTKSWNFIGFDETSKXTTYE, which is 60% identical with subtilisin-like serine protease from Narcissus pseudonarcissus.


  • 주제어

    Allium tuberosum .   fibrinolytic enzyme .   fibrin zymography .   subtilisin-like.  

  • 참고문헌 (29)

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  • 이 논문을 인용한 문헌 (2)

    1. 2010. "" Journal of medicinal food, 13(6): 1532~1536     
    2. 2013. "" Food science and biotechnology, 22(5): 1367~1373     

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