본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Food science and biotechnology v.19 no.3, 2010년, pp.757 - 762   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Biochemical Characterization of Dextranase from Arthrobacter oxydans and Its Cloning and Expression in Escherichia coli

Lee, Jin-Ha    (Dental Science Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University   ); Nam, Seong-Hee    (Jeonnam Agricultural Research and Extension Services   ); Park, Hyen-Joung    (Cosmax Ltd.   ); Kim, Young-Min    (School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University   ); Kim, Na-Hyun    (Korean Minjok Leadership Academy   ); Kim, Gha-Hyun    (Biochemistry and Cell Biology, University of California-San Diego   ); Seo, Eun-Seong    (School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University   ); Kang, Seong-Soo    (College of Veterinary Medicine, Chonnam National University   ); Kim, Do-Man    (School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University  );
  • 초록

    Appreciably elevated levels of dextranase from Arthrobacter oxydans (AODex) isolated from sugar-cane farm soil was resulted from the culture on the Luria-Bertani (LB) medium containing 1%(w/v) soluble starch, glycerol, or dextran. The responsible gene (aodex) was cloned, its nucleotide sequence was determined, and expression of the encoded protein was achieved in Escherichia coli. An open reading frame was composed of 1,863 bp putatively encoding a 68.3 kDa protein. Recombinant A. oxydans dextranase (rAODex) was purified about 16 fold by nickel-nitrilotriacetic acid affinity column chromatography; $K_m$ value for dextran T2000 was 0.85 mg/mL (w/v). AODex treatment of stale sugar cane juice resulted in a yield of square and light-colored sugar crystals.


  • 주제어

    dextranase .   Arthrobacter oxydans .   dextran .   sugar .   expression.  

  • 참고문헌 (20)

    1. Khalikova E, Susi P, Korpela T. Microbial dextran-hydrolyzing enzymes: Fundamentals and applications. Microbiol. Mol. Biol. R. 69: 306-325 (2005) 
    2. Coutinho PM, Henrissat B. Carbhydrate-active enzymes: An integrated database approach. pp. 3-12. In: Recent Advances in Carbohydrate Bioengineering. Gilbert HJ, Davies GJ, Henrissat B, Svensson B (eds). The Royal Society of Chemistry, Cambridge, UK (1999) 
    3. Oguma T, Kurokawa T, Tobe K, Kitao S, Kobayashi M. Cloning and sequence analysis of the gene for glucodextranase from Arthrobacter globiformis T-3044 and expression in E. coli cells. Biosci. Biotech. Bioch. 63: 2174-2182 (1999) 
    4. Kang HK, Kim SH, Park JY, Jin XJ, Oh DK, Kang SS, Kim D. Cloning and characterization of dextranase gene (LSD1) from Lipomyces starkeyi and its expression in Saccharomyces cerevisae. Yeast 22: 1239-1248 (2005) 
    5. Garcia B, Margolles E, Roca H, Mateu D, Raices M, Gonzales ME, Herrera L, Delgado J. Cloning and sequencing of dextranaseencoding cDNA from Penicillium minioluteum. FEBS Microbiol. Lett. 143: 175-183 (1996) 
    6. Mizuno T, Mori H, Ito H, Matsui H, Kimura A, Chiba S. Molecular cloning of isomaltotrio-dextranase gene from Brevibacterium fuscum var. dextranlyticum strain 0407 and its expression in E. coli. Biosci. Biotech. Bioch. 63: 1582-1588 (1999) 
    7. Tonozuka T, Suzuki S, Ikehara Y, Mizuno M, Kim YK, Nishikawa A, Sakano Y. Heterogonous production and characterization of Arthrobacter globiformis T6 isomalto-dextranase. J. Appl. Glycosci. 51: 27-32 (2004) 
    8. Molodova GA, Danilova TI, Maksimov VI, Kozhevnikova NP. Methods of purifying dextranase from Penicillium funiculosum. Prikl. Biokhim. Mikrobiol. 16: 909-914 (1980) 
    9. Kim D, Day DF. A new process for the production of clinical dextran by mixed-culture fermentation of Lipomyces starkeyi and Leuconostoc mesenteroides. Enzyme Microb. Tech. 16: 844-848 (1994) 
    10. Lee JH, Kim GH, Kim SH, Cho DL, Kim DW, Day DF, Kim D. Treatment with glucanohydrolase from Lipomyces starkeyi for removal of soluble polysaccharides in sugar processing. J. Microbiol. Biotechn. 16: 983-987 (2006)     
    11. Hatada Y, Hidaka Y, Nogi Y, Uchimura K, Katayama K, Li Z, Akita M, Ohta Y, Goda S, Ito H, Matsui H, Ito S, Horikoshi K. Hyperproduction of an isomalto-dextranase of an Arthrobacter sp. by a protease-deficient Bacilllus subtilis: Sequencing, properties, and crystallization of the recombinant enzyme. Appl. Microbiol. Biotechnol. 65: 583-592 (2004) 
    12. Iwai A, Ito H, Mizuno T, Mori H, Matsui H, Honma M, Okada G, Chiba S. Molecular cloning and expression of an isomaltodextranase gene from Arthrobacter globiformis T6. J. Bacteriol. 176: 7730-7734 (1994) 
    13. Okada G, Takayanagi T, Sawai T. Improved purification and further characterization of an isomaltodextranase from Arthrobacter globiformis T6. Agr. Biol. Chem. Tokyo 52: 495-501 (1988) 
    14. Kubo S, Kubota H, Ohnishi Y, Morita T, Matsuya T, Matsushiro A. Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii. Infect. Immun. 61: 4375-4381 (1993) 
    15. Fox JD, Robyt J. Miniaturization of three carbohydrate analyses using a microsample plate reader. Anal. Biochem. 195: 93-96 (1991) 
    16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 (1976) 
    17. Ohnishi Y, Kubo S, Ono Y, Nozaki M, Gonda Y, Okano H, Matsuya T, Matsushiro A, Morita T. Cloning and sequencing of the gene coding for dextranase from Streptococcus salivarius. Gene 14: 93-96 (1995) 
    18. Ryu HJ, Kim D, Kim DW, Moon YY, Robyt F. Cloning of a dextransucrase gene (fmcmds) from a constitutive dextransucrase hyper-producing Leuconostoc mesenteroides B-512FMCM developed using VUV. Biotechnol. Lett. 22: 421-425 (2000) 
    19. Igarashi T, Morisaki H, Goto N. Molecular characterization of dextranase from Streptococcus rattus. Microbiol. Immunol. 48: 155-162 (2004) 
    20. Okushima M, Sugino D, Kouno Y, Nakano S, Miyahara J, Toda H, Kubo S, Matsushiro A. Molecular cloning and nucleotide sequencing of the Arthrobacter dextranase gene and its expression in Escherichia coli and Streptococcus sanguis. Jpn. J. Genet. 66: 173-187 (1991) 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기