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Food science and biotechnology v.19 no.3, 2010년, pp.809 - 813   SCIE
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Identification of Estrone in Pomegranate (Punica granatum) Extracts by Liquid Chromatography-Tandem Mass Spectrometry

Kho, Young-Lim    (School of Human and Environmental Sciences, Eulji University   ); Jung, Woong    (Department of Emergency Medicine, East-West Neo Medical Center, Kyung Hee University   ); Kwon, Do-Hyeong    (Research Center, Hanil PFC Co., Ltd.   ); Kim, Jung-Hoan    (School of Natural Food Sciences, Eulji University  );
  • 초록

    There were some reports that the pomegranate juice, seeds, and peel extracts contain steroid hormones including estrone, but the results were controversial. This study was performed to identify estrone in pomegranate extracts through a more sensitive and validated technology. Separation and determination of estrone was carried out using high performance liquid chromatography (HPLC) system with triple quadrupole mass spectrometry. Low concentration of estrone ( $4.5{\pm}0.38\;ng/mL$ ) was detected in pomegranate extracts with adequate precision value. The amount of estrone in pomegranate extracts was calculated as 16.4, 18.2, and 20.2 ng/mL, respectively, adjusted by extraction efficiency (average 25.6%) in the precision verification.


  • 주제어

    pomegranate .   estrone .   estrogen .   liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) .   phytochemical.  

  • 참고문헌 (19)

    1. Longtin R. The pomegranate: Nature's power fruit. J. Natl. Cancer I. 95: 346-348 (2003) 
    2. Schubert SY, Lansky EP, Neeman I. Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J. Ethnopharmacol. 66: 11-17 (1999) 
    3. Braga LC, Leite AA. Xavier KG. Synergistic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can. J. Microbiol. 51: 541-547 (2005) 
    4. Zhanag J, Zhan B, Yao X, Gao Y, Shong J. Antiviral activity of tannin from the pericarp of Punica granatum L. against genital Herpes virus in vitro. Zhongguo Zhong Yao Za Zhi 20: 556-558 (1995) 
    5. Jeune MA, Kumi-Diaka J, Brown J. Anticancer activities of pomegranate extracts and genistein in human breast cancer cells. J. Med. Food 8: 469-475 (2005) 
    6. Kim ND, Mehta B, Yu W, Neeman I, Livney T, Amichay A, Poirier D, Nicholls P, Kirby A, Jiang W, Mansel R, Ramachandran C, Rabi T, Kaplan B, Lansky E. Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. Breast Cancer Res. Tr. 71: 203-217 (2002) 
    7. Espin JS, Garcia-Conesa MT, Tomas-Barberan FA. Nutraceuticals: Facts and fiction. Phytochemistry 68: 2986-3008 (2007) 
    8. Gil MI, Garcia-Viguera C, Artes F, Tomas-Barberan FA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Sci. Food Agr. 68: 77-81 (1995) 
    9. Lee JH, Kim SD, Lee JY, Kim KN, Kim HS. Analysis of flavonoids in concentrated pomegranate extracts by HPLC with diode array detection. Food Sci. Biotechnol. 14: 171-174 (2005)     
    10. Melgarejo P, Artes F. Total lipid content and fatty acid composition of oilseed from lesser known sweet pomegranate clones. J. Sci. Food Agr. 80: 1452-1454 (2000) 
    11. Hopkins CY, Chrisholm MJ. A survey of the conjugated fatty acids of seed oils. J. Am. Oil Chem. Soc. 45: 176-182 (1968) 
    12. Heftmann E, Ko ST, Bennet RD. Identification of estrone in pomegranate seeds. Phytochemistry 5: 1337-1340 (1966) 
    13. Choi DW, Kim JY, Choi SH, Jung HS, Kim HJ, Cho HJ, Cho SY, Kang CS, Chang SY. Identification of steroid hormones in pomegranate (Punica granatum) using HPLC and GC-mass spectrometry. Food Chem. 96: 562-571 (2006) 
    14. Tong P, Kasuga Y, Khoo CS. Liquid chromatographic-mass spectrometric method for detection of estrogen in commercial oils and in fruit seed oils. J. Food Compos. Anal. 19: 150-156 (2006) 
    15. Elswijk DA, Schobel UP, Lansky EP, Irth H, Greef J. Rapid dereplication of estrogenic compounds in pomegranate (Punica granatum) using on-line biochemical detection coupled to mass spectrometry. Phytochemistry 65: 233-241 (2004) 
    16. Farre M, Kuster M, Brix R, Rubio F, Alda ML, Barcelo D. Comparative study of an estradiol enzyme-linked immunosorbent assay kit, liquid chromatography-tandem mass spectrometry, and ultra performance liquid chromatography-quadrupole time of flight mass spectrometry for part-per-trillion analysis of estrogens in water samples. J. Chromatogr. A 1160: 166-175 (2007) 
    17. Hosogia J, Tanaka H, Fujita K, Kuwabara T, Ikegawa S, Kobayashi N, Mano N, Goto J. LC-MS/MS coupled with immunoaffinity extraction for determination of estrone, 17$\beta$-estradiol, and estrone 3- sulfate in human plasma. J. Chromatogr. B 878: 222-227 (2010) 
    18. US EPA. Revised Assessment of Detection and Quantitation Approaches. EPA-821-B-04-005. Office of Water, US Environmental Protection Agency, Washington, DC, USA (2004) 
    19. Anesini C, Perez C. Screening of plants used in Argentine folk medicine for antimicrobial activity. J. Ethnopharmacol. 39: 119-128 (1993) 

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