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Food science and biotechnology v.19 no.3, 2010년, pp.821 - 826   SCIE 피인용횟수: 3
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Anthocyanins in Cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]

Ha, Tae-Joung    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Lee, Myoung-Hee    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Jeong, Yu-Na    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Lee, Jin-Hwan    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Han, Sang-Ik    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Park, Chang-Hwan    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Pae, Suk-Bok    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Hwang, Chung-Dong    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Baek, In-Youl    (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration   ); Park, Keum-Yong    (Department of Funct  );
  • 초록

    The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black cowpea with 1% trifluoroacetic acid (TFA) in methanol, isolated by RP-C-18 column chromatography, and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The isolated anthocyanins were characterized as delphinidin-3-O-glucoside (2) and cyanidin-3-Oglucoside (4). Furthermore, 5 minor anthocyanins were detected and identified as delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (5), peonidin-3-O-glucoside (7), and malvidin-3-O-glucoside (8) based on the fragmentation patterns of HPLC-DAD-ESI/MS analysis.


  • 주제어

    Vigna unguiculata .   cowpea .   anthocyanin .   high performance liquid chromatography.  

  • 참고문헌 (23)

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  • 이 논문을 인용한 문헌 (3)

    1. Jung, Yang Sook ; Choi, Kyung-Jin ; Kang, Hang-Won ; Bae, Do-Gyu 2011. "Research on the Colorants Extraction from Black Cowpea Seed Coats and their Storage Stability" 韓國資源植物學會誌 = Korean journal of plant resources, 24(5): 499~506     
    2. Kim, Dong-Kwan ; Son, Dong-Mo ; Choi, Jin-Gyung ; Shin, Hae-Ryong ; Choi, Kyeong-Ju ; Lee, Jeongran ; Lee, Kyung-Dong ; Rim, Yo-Sup 2013. "Agronomic Characteristics and Seed Quality of Cowpea (Vigna unguiculata L.) Germplasm" Korean journal of crop science = 韓國作物學會誌, 58(1): 1~7     
    3. 2014. "" Fibers and polymers, 15(1): 138~144     

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