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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology v.14 no.3, 2010년, pp.133 - 137   SCIE 피인용횟수: 1
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Stimulatory Effects of Ginsan on the Proliferation and Viability of Mouse Spleen Cells

Ko, Eun-Ju    (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University   ); Joo, Hong-Gu    (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University  );
  • 초록

    Ginsan is an acidic polysaccharide purified from Panax ginseng, a famous oriental herb. Although a variety of biological activities of ginsan have been studied, the effects of ginsan on spleen cells are not fully elucidated. We investigated the effect of ginsan on the viability and proliferation of spleen cells. Using Cell Counting $Kit-8^{(R)}$ solution and trypan blue solution, we found that ginsan significantly enhanced viability and proliferation. Multiple clusters, indicating proliferation, were observed in ginsan-treated spleen cells and, carboxyfluorescein succinimidyl ester and surface marker staining assay revealed that ginsan promoted proliferation from $CD19^+$ B cells rather than $CD4^+$ or $CD8^+$ T cells. In addition, ginsan decreased the percentage of late apoptotic cells. Ginsan increased the surface expression of CD25 and CD69 as well as production of interleukin-2 from spleen cells, suggesting increased activation. Taken together, these results demonstrate that ginsan increases the viability and proliferation of spleen cells via multiple mechanisms, valuable information for broadening the use of ginsan in clinical and research settings.


  • 주제어

    B lymphocytes .   Ginsan .   Stimulation .   Proliferation .   Spleen cells.  

  • 참고문헌 (18)

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  • 이 논문을 인용한 문헌 (1)

    1. 2016. "" Asian Pacific journal of cancer prevention : APJCP, 17(7): 3559~3567   

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