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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology v.14 no.3, 2010년, pp.169 - 176   SCIE
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Hyperosmotic Stimulus Down-regulates $1{\alpha}$, 25-dihydroxyvitamin $D_3$-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System

Tian, Yu-Shun    (Department of Oral Biology, Yonsei University   ); Jeong, Hyun-Joo    (Department of Oral Biology, Yonsei University   ); Lee, Sang-Do    (Department of Physiology, College of Medicine, Chungnam National University   ); Kong, Seok-Heui    (Department of Orthodontics, Yonsei University   ); Ohk, Seung-Ho    (Department of Oral Microbiology, College of Dentistry, Chonnam National University   ); Yoo, Yun-Jung    (Department of Oral Biology, Yonsei University   ); Seo, Jeong-Taeg    (Department of Oral Biology, Yonsei University   ); Shin, Dong-Min    (Department of Oral Biology, Yonsei University   ); Sohn, Byung-Wha    (Department of Orthodontics, Yonsei University   ); Lee, Syng-Ill    (Department of Oral Biology, Yonsei University  );
  • 초록

    The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects $1{\alpha}$ , 25-dihydroxyvitamin $D_3$ ( $1{\alpha},25(OH)_2D_3$ )-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on $1{\alpha},25(OH)_2D_3$ -induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of $1{\alpha},25(OH)_2D_3$ -induced tartrate-resistant acid phosphatase-positive multinucleated cells and $1{\alpha},25(OH)_2D_3$ -induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) and Runx2 expressions were down-regulated in response to $1{\alpha},25(OH)_2D_3$ . Knockdown of Runx2 inhibited $1{\alpha},25(OH)_2D_3$ -induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of $1{\alpha},25(OH)_2D_3$ -induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.

  • 주제어

    Hyperosmotic stimulus .   TonEBP .   Osteoblast .   RANKL .   Runx2.  

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