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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology v.14 no.3, 2010년, pp.177 - 183   SCIE 피인용횟수: 2
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Tetrahydrobiopterin Inhibits PDGF-stimulated Migration and Proliferation in Rat Aortic Smooth Muscle Cells via the Nitric Oxide Synthase-independent Pathway

Jiang, Xiaowen    (Department of Physiology and Biotechnology, Konkuk University   ); Kim, Bo-Kyung    (Department of Physiology and Biotechnology, Konkuk University   ); Lin, Haiyue    (Department of Physiology and Biotechnology, Konkuk University   ); Lee, Chang-Kwon    (Department of Physiology and Biotechnology, Konkuk University   ); Kim, Jung-Hwan    (Department of Physiology and Biotechnology, Konkuk University   ); Kang, Hyun    (Department of Physiology and Biotechnology, Konkuk University   ); Lee, Pil-Young    (Department of Physiology and Biotechnology, Konkuk University   ); Jung, Seung-Hyo    (Department of Physiology and Biotechnology, Konkuk University   ); Lee, Hwan-Myung    (Department of Cosmetic Science, College of Natural Science, Hoseo University   ); Won, Kyung-Jong    (Department of Physiology and Biotechnology, Konkuk University  );
  • 초록

    Tetrahydrobiopterin (BH4), an essential cofactor for nitric oxide synthase (NOS) activity, is known to play important roles in modulating both NO and superoxide production during vascular diseases such as atherosclerosis. However, the role of BH4 in functions of vascular smooth muscle cells is not fully known. In this study, we tested the effects of BH4 and dihydrobiopterin (BH2), a BH4 precursor, on migration and proliferation in response to platelet-derived growth factor-BB (PDGF-BB) in rat aortic smooth muscle cells (RASMCs). Cell migration and proliferation were measured using a Boyden chamber and a 5-bromo-2'-deoxyuridine incorporation assay, respectively, and these results were confirmed with an ex vivo aortic sprout assay. Cell viability was examined by 2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide assays. BH4 and BH2 decreased PDGF-BBinduced cell migration and proliferation in a dose-dependent manner. The inhibition of cell migration and proliferation by BH4 and BH2 was not affected by pretreatment with $N^G$ -nitro-L-arginine methyl ester, a NOS inhibitor. Moreover, the sprout outgrowth formation of aortic rings induced by PDGF-BB was inhibited by BH4 and BH2. Cell viability was not inhibited by BH4 and BH2 treatment. The present results suggest that BH4 and BH2 may inhibit PDGF-stimulated RASMC migration and proliferation via the NOS-independent pathway. Therefore, BH4 and its derivative could be useful for the development of a candidate molecule with an NO-independent anti-atherosclerotic function.


  • 주제어

    Tetrahydrobiopterin .   Migration .   Nitric oxide .   Proliferation .   Rat aortic smooth muscle cells.  

  • 참고문헌 (30)

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  • 이 논문을 인용한 문헌 (2)

    1. 2011. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 34(9): 1571~1577     
    2. Kim, Min-Ju ; Kim, Ae-Jung 2015. "Biological Activity and Manufacturing of Yanggeng with Yangha Flower Buds" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 44(8): 1180~1185     

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