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Molecular & cellular toxicology v.6 no.2, 2010년, pp.127 - 133   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Heme oxygenase-1 mediated protective effect of methyl gallate on cadmium-induced cytotoxicity in cultured mouse mesangial cells

Cha, Seok-Ho    (Department of Medical Biotechnology, College of Medicine, Inha University   ); Suh, Chang-Kook    (Department of Physiology and Biophysics, College of Medicine, Inha University  );
  • 초록

    To clarify the effect of a phytochemical, methyl gallate (MG) on a heavy metal (cadmium)-induced renal toxicity, cytotoxicity and the change of heme oxygenase-1 (HO-1) gene expression was studied using cultured mouse renal glomerular mesangial cells (MMC). By employing RT-PCR and Western blotting analysis, we have examined the HO-1 induction in MMCs that were treated with $Cd^{2+}$ and/or MG. Using MTT assay we have also examined the cytoprotective effect of HO-1 induction against the cytotoxicity caused by toxic dose of $Cd^{2+}$ . In MMCs exposed to $Cd^{2+}$ and MG, expression of HO-1 (mRNA and protein) was increased in a concentration- and time-dependent manner. The increments of HO-1 mRNA and protein expressions by $Cd^{2+}$ and MG were inhibited by the treatment of the cells with actinomycin D, an inhibitor of transcription. The decreased viability of the cells by $Cd^{2+}$ was partially recovered by the treatment of MG and this recovery by the MG was reduced by the treatment of zinc protoporphyrin IX (a HO-1 inhibitor). From these results, methyl gallate might have cytoprotective effect on $Cd^{2+}$ -induced cytotoxicity that is related with heme oxygenase-1 induction.


  • 주제어

    Heme oxygenase-1 .   Cadmium .   Mouse mesangial cell .   Methyl gallate.  

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