본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of crop science and biotechnology v.13 no.2, 2010년, pp.107 - 112  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

High Frequency Direct Plant Regeneration from Leaf and Petals of Cape Primrose (Streptocarpus)

Chaudhury, A.    (Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology   ); Power, J.B.    (Division of Crop and Plant Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus   ); Davey, M.R.    (Division of Crop and Plant Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus  );
  • 초록

    High frequency direct plant regeneration from leaf and petal explants was accomplished for the first time in Streptocarpus varieties. The shoot induction frequency varied with respect to the benzylaminopurine (BAP) concentration added to the Murashige and Skoog (MS) medium. MS medium with $0.5\;mg\;l^{-1}$ BAP exhibited the highest (69.9%) plant regeneration frequency with an average of 186 shoots per explant. A higher concentration of BAP inhibited shoot bud induction and plant regeneration along with necrosis of explants. Petal explants derived from the varieties 'Branwen' (pink and white) and 'Chorus Line' (violet and white) displayed plant regeneration frequency of 22.2-47.4% (within a total of 12 weeks) on MS medium containing $2.0\;mg\;l^{-1}$ $\alpha$ -naphthaleneacetic acid and $0.5\;mg\;l^{-1}$ BAP for 8 weeks followed by 4 weeks on MS medium with $1.0\;mg\;l^{-1}$ BAP. Scanning electron microscopy confirmed direct plant regeneration without callus. Regenerated plants from leaf explants with well-developed leaves and roots were hardened and successfully transferred to pots in glasshouse exhibiting 86% survival at the end of 4-6 weeks. Whereas, regenerated plants from flower petal explants upon transfer to pots in glasshouse exhibited 75-82% survival at the end of 4-6 weeks.


  • 주제어

    cluster .   direct plant regeneration .   flower petal .   Streptocarpus.  

  • 참고문헌 (20)

    1. Appelgren M, Heide OM. 1972. Regeneration in Streptocarpus leaf discs and its regulation by temperature and growth sub stances. Physiol. Plant. 27:417-423 
    2. Bailey LH, Bailey EZ. 1976. Streptocarpus, In H Third, ed, A Concise Dictionary of Plants Cultivated in the United Satesand Canada, Macmillan Publishing, New York, pp, 1079-1088 
    3. Chen TY, Chen JT, Chang WC. 2004. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell, Tissue Org. Cult. 76:11-15 
    4. Floh EIS, Handro W. 2001. Effect of photoperiod and chloro genic acid on morphogenesis in leaf discs of Streptocarpus nobilis. Biol. Plant. 44:615-618 
    5. Handro W. 1976. On flower initiation in Streptocarpus nobilis CB Clarke (Gesneriaceae). Biologia Botanica University S. Paulo 4:31-39 
    6. Handro W. 1977a. Structural aspects of neo-formation of floral buds on leaf discs of Streptocarpus nobilis cultured in vitro. Annals Bot. 41:303-305 
    7. Handro W. 1977b. Photoperiodic induction of flowering on different explanted tissues from Streptocarpus nobilis cultured in vitro. Biol. Bot. University S. Paulo 5:21-26 
    8. Handro W, Monteiro-Scanavacca WR. 1978. Changes in the shoot apex of Streptocarpus nobilis under conditions of photoperiodic induction. Can. J. Bot. 56:365-369 
    9. Harrison CJ, Moller M, Cronk QCB. 1999. Evolution and devel opment of floral diversity in Streptocarpus and Saintpaulia. Annals Bot. 84:49-60 
    10. Hilliard OM, Burt BL. 1971. Streptocmpus: An African plant study. Pp.410. Pietermaritzburg, South Africa, University of NatalPress Hughes M, Moller M, Bellstedt DU, Edwards TJ, Woodhead M. 2004. EST and random genomic nuclear microsatellite markers for Streptocarpus. Mol. Ecol. Notes 4:36-38 
    11. Martin KP, Dominic J, Madassery J, Philip VJ. 2003. Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. In Vitro Cell Devel. Biol. Plant 39:500-504 
    12. Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473-479 
    13. Nitsch JP. 1967. Streptocarpus nobilis, plant de jours courts. Bull. Soc. Bot. France 114:128-132 
    14. Peck DE, Cumming BG. 1984. In vitro vegetative propagation of Cape Primrose using corolla of the flower. Hortscience 19:339-340 
    15. Raman K. 1977. Rapid multiplication of Streptocarpus and Gloxinia from in vitro cultured pedicel segments. Zeitschrift fur Pflanzenphysiol. 83:411-418 
    16. Rossini LME, Nitsch JP. 1966. Induction de la floraison in vitro chez une plant de jours courts, Streptocarpus nobilis. CR Acad. Sci. Series D 263:1379-1382 
    17. Simmonds J. 1982. In vitro flowering on leaf tissue of Streptocarpus nobilis. The influence of culture medium com ponents on vegetative and reproductive development. Can. J. Bot. 60:1461-1468 
    18. Simmonds J. 1985. In vitro photo induction of leaf tissue of Streptocarpus nobilis. Biologia Plant. 27:318-324 
    19. Verhoeven JTJ, Bouwen I, Roenhorst JW. 1995. A new flower breaking tobamovirus of Streptocarpus. Eur. J. Plant Pathol. 101:311-318 
    20. Wolff DW, Veilleux RE, Jensen CJ. 1986. Evaluation of antherderived Streptocarpus hybridus and their progeny. Plant Cell Tissue Org. Cult. 6:167-172 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기