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Food science and biotechnology v.20 no.1, 2011년, pp.123 - 129   SCIE SCOPUS
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Determination of Biogenic Amines in Cheonggukjang Using Ultra High Pressure Liquid Chromatography Coupled with Mass Spectrometry

Lee, Sang-Hee    (Korea Food Research Institute   ); Eom, Hye-Seon    (Korea Food Research Institute   ); Yoo, Mi-Young    (Korea Food Research Institute   ); Cho, Yong-Sun    (Korea Food Research Institute   ); Shin, Dong-Bin    (Korea Food Research Institute  );
  • 초록

    A rapid and reliable method for the simultaneous determination of 12 biogenic amines (BAs; agmatine, phenylethylamine, tyramine, putrescine, cadaverine, histamine, serotonin, tryptamine, spermidine, noreinephrine, dopamine, and spermine) in cheonggukjang was optimized and validated using ultra high pressure liquid chromatography-electrospray tandem mass spectrometry (UHPLCESI-MS/MS). The BAs were dansylated and separated on a C8 column under LC gradient of 7 min duration, and detected by MS/MS with the multiple reaction monitoring mode (MRM). This method exhibited excellent linearity for all of the analytes with correlation determination ( $R^2$ ) higher than 0.98. The limits of detection (LODs) were $10.8-39.6{\mu}g/kg$ . The precision results were expressed as relative standard deviation (RSD), ranged from 0.3 to 14.3% for intra-day and from 0.9 to 15.4% for the interday. The proposed method will help to ensure for quality control by monitoring of BAs in cheonggukjang.


  • 주제어

    biogenic amine .   cheonggukjang .   ultra high pressure liquid chromatography-electrospray tandem mass spectrometry (UHPLC-ESI-MS/MS) .   validation.  

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