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Food science and biotechnology v.20 no.1, 2011년, pp.201 - 207   SCIE SCOPUS 피인용횟수: 1
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Rapid and Simple Biochemical Detection for Salmonella spp. Using Modified LB Broth and the MUCAP Test

Jang, Keum-Il    (Department of Food Science and Technology, Chungbuk National University   ); Ahn, Jun-Bae    (Department of Food Service Industry, Seowon University   ); Kim, Kwang-Yup    (Department of Food Science and Technology, Chungbuk National University  );
  • 초록

    In this study, we developed a rapid Salmonella detection system and confirmed its detection efficiency using modified selective pre-enrichment and the 4-methyl umbelliferyl caprilate (MUCAP) test. Luria-Bertani (LB) medium without auto-fluorescence was modified by the addition of a bile salt mixture and sodium citrate as inhibitors, after which ferrous sulfate and sodium thiosulfate were added for the induction of $H_2S$ gas. In a mixed culture of Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis in Salmonella detective modified LB (SDMLB) medium, S. enteritidis dominated regardless of their initial population size. Among 10 bacterial strains, Salmonella and Enterobacter spp. were cultured and distinguished by the $H_2S$ test because the Enterobacter spp. was unable to produce $H_2S$ gas in SDMLB medium. MUCAP testing produced blue fluorescence only for Salmonella spp. Tests for Salmonella using chicken skin and egg shells revealed the presence of Salmonella spp. Thus, foods carrying Salmonellae were detected rapidly and simply using this method.


  • 주제어

    Salmonella .   $H_2S$ test .   4-methyl umbelliferyl caprilate (MUCAP) test .   Salmonella detective modified LB (SDMLB) medium .   rapid detection.  

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  • 이 논문을 인용한 문헌 (1)

    1. Lee, Siwon ; Park, Jungan ; Lee, O-Mi ; Shin, Yong-Gil 2013. "Plant quarantine isolated cultivation system in Korea and results of recorded in 2005-2012" 농업과학연구 = CNU Journal of agricultural science, 40(4): 281~287     

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