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Food science and biotechnology v.20 no.1, 2011년, pp.277 - 281   SCIE SCOPUS
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Cloning of Levansucrase from Leuconostoc mesenteroides and Its Expression in Pichia pastoris

Kang, Hee-Kyoung    (The Research Institute for Catalysis, Chonnam National University   ); Yun, Sang-Il    (School of Biological Sciences and Technology, Chonnam National University   ); Lim, Tae-Yoon    (Department of Biological Science, Korea Advanced Institute of Science and Technology (KAIST)   ); Xia, Yong-Mei    (State Key Laboratory of Food Science and Technology, Jiangnan University   ); Kim, Do-Man    (The Research Institute for Catalysis, Chonnam National University  );
  • 초록

    A gene (m1ft) encoding levansucrase from Leuconostoc mesenteroides has been previously cloned in Escherichia coli. Presently, m1ft was cloned and secretively expressed in Pichia pastoris. Methanol induction of recombinant M1FT resulted in the production of active levansucrase (PM1FT). PM1FT-5 was expressed as an active form, but the protein accumulated mainly inside the cells, representing about 5% of total cell proteins. M1FT was secreted into the culture supernatant with a maximum yield of 14,400 U/L using fed-batch fermentations. P. pastoris-derived M1FT displayed catalytic activities comparable to those of the E. coli-derived M1FT. PM1FT was glycosylated at its 2 potential N-glycosylation sites.


  • 주제어

    levansucrase .   Leuconostoc mesenteroides .   Pichia pastoris .   expression .   levan.  

  • 참고문헌 (20)

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