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The Transcription Factor Mist1 Regulates the Cellular Polarity in Mouse Pancreatic Acinar Cells

Yang, Yu-Mi    (Department of Oral Biology, Yonsei University College of Dentistry   ); Lee, Syng-Ill    (Department of Oral Biology, Yonsei University College of Dentistry   ); Shin, Dong-Min    (Department of Oral Biology, Yonsei University College of Dentistry  );
  • 초록

    Pancreatic acinar cells exhibit a polarity that is characterized by the localization of secretory granules at the apical membrane. However, the factors that regulate cellular polarity in these cells are not well understood. In this study, we investigated the effect of Mist1, a basic helix-loop-helix transcription factor, on the cellular architecture of pancreatic acinar cells. Mist1-null mice displayed secretory granules that were diffuse throughout the pancreatic acinar cells, from the apical to basolateral membranes, whereas Mist1 heterozygote mice showed apical localization of secretory granules. Deletion of the Mist1 gene decreased the expression of type 3 inositol 1,4,5-triphosphate receptors ( $IP_3R$ ) but did not affect apical localization and expression of $IP_3R2$ . Mist1-null mice also displayed an increase in luminal areas and an increase in the expression of zymogen granules in pancreatic acinar cells. These results suggest that Mist1 plays a critical role in polar localization of cellular organelles and in maintaining cellular architecture in mouse pancreatic acinar cells.


  • 주제어

    cell polarity .   Mist1 .   secretory vesicles .   pancreatic acinar cells.  

  • 참고문헌 (17)

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 저자의 다른 논문

  • Yang, Yu-Mi (1)

    1. 2013 "Fast temporal detection of intracellular hydrogen peroxide by HyPer" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute 38 (4): 169~173    
  • Lee, Syng-Ill (17)

  • Shin, Dong-Min (7)

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