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생약학회지 v.43 no.1 = no.168, 2012년, pp.39 - 45   피인용횟수: 3

엉겅퀴 70% 에탄올 추출물의 RAW264.7 세포에서 Heme oxygenase-1 발현을 통한 항염증 효과
Anti-inflammatory Effect of the Cirsium japonicum var. ussuriense 70% Ethanolic Extract in RAW264.7 Cells by Heme oxygenase-1 Expression

이동성    (원광대학교 약학대학   ); 김경수    (원광대학교 약학대학   ); 리빈    (원광대학교 약학대학   ); 최현규    (원광대학교 약학대학   ); 케오썸엘    (원광대학교 약학대학   ); 전기용    (원광대학교 약학대학   ); 박준형    (원광대학교 약학대학   ); 김윤철    (원광대학교 약학대학  );
  • 초록

    Cirsium japonicum var. ussuriense has long been used in herbal medicine for the treatment of arthritis, dyspepsia, and bleeding in Korea. In the present study, we investigated anti-inflammatory effects of C. japonicum var. ussuriense against lipopolysaccharide(LPS)-induced activation in RAW264.7 cells by the expression of heme oxygenase (HO)-1. The 70% EtOH extract of the aerial parts of C. japonicum var. ussuriense (CJE), showed the potent anti-inflammatory effects on LPS-induced inflammation in RAW264.7 cells. The anti-inflammatory effect of CJE was demonstrated by the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2). Furthermore CJE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increased HO activity in RAW264.7 macrophages. The effects of CJE on LPS-induced NO and $PGE_2$ productions were partially reversed by an HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that CJE-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. These results suggest that CJE may be a promising candidate for the treatment of inflammatory diseases.


  • 주제어

    Cirsium japonicum var.   ussuriense .   Heme oxygenase-1 .   RAW264.   7 .   iNOS .   COX-2 .   Inflammation.  

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  • 이 논문을 인용한 문헌 (3)

    1. Kim, Ma-Ryong ; Kang, Ok-Hwa ; Kim, Sung-Bae ; Kang, Hee-Jung ; Kim, Ji-Eun ; Hwang, Hyeong-Chil ; Kim, In-Won ; Kwon, Dong-Yeul 2013. "The Study of Anti-inflammatory Effect of Hwanggeumjakyak-tang Extract in RAW 264.7 Macrophage" 大韓本草學會誌 = The Korea journal of herbology, 28(1): 43~50     
    2. Kim, Seon-Young ; Kim, Sang-Jun ; Choi, Young Ji ; Yu, Kang-Yeol ; Chung, Chang-Ho ; Shim, Jae-Suk ; Jang, Seon-Il ; Yu, Dong-Hyun ; Jeong, Seung-Il 2013. "Analysis of Active Components in Cirsium japonicum var. ussuriense Extracts and Their Effect on TGF-beta Induced Hepatic Stellate Cells Activation" 생약학회지, 44(2): 110~117     
    3. Jang, Miran ; Park, Hyejin ; Hong, Eunyeong ; Kim, Gun-Hee 2014. "Comparison of the Antibacterial Activity of Domestic Cirsium japonicum Collected from Different Regions" 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 30(3): 278~283     

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