본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Korean journal for food science of animal resources = 한국축산식품학회지 v.32 no.2, 2012년, pp.247 - 251   SCIE SCOPUS
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Quantitative Analysis of Central Nervous System Tissues (CNST) in Beef By-Products in Retail Market

Lim, Dong-Gyun    (Department of Agricultural Biotechnology, Seoul National University   ); Lee, Moo-Ha    (Department of Agricultural Biotechnology, Seoul National University  );
  • 초록

    This study was carried out to investigate the applicability of the detection of central nervous system tissues (CNST) in beef by-products in retail market. Beef by-products including large intestine, brain, spinal cord, liver, lung, spleen and heart were purchased and tested for the presence of CNST using an ELISA method. The ELISA test was evaluated and showed a high correlation coefficient by a standard curve (R value = 0.999). Based on the analytical instruction, the positive indication of the CNST contamination of brain and spinal cord was detected above 0.1% but large intestine, liver, lung, spleen, and heart was negative. Result suggests that the ELISA method is applicable to a real meat system and may provide a method to ensure confidence for consumer against bovine spongiform encephalopathy (BSE).

  • 주제어

    beef by-products .   central nervous system tissue .   ELISA.  

  • 참고문헌 (28)

    1. Almond, J., and Pattison, J. (1997) Human BSE. Nature 389, 437-438. 
    2. Anonymous. (1999) SigmaPlot for Windows Version 5.00, SPSS INC., U.S.A. 
    3. Anil, M. H., Love, S., Williams, S., Shand, A., Mckinstry, J. L., Helps, C. R., Waterman-Pearson, A., Seghatchian, J., and Harbour, D. A. (1999) Potential contamination of beef carcasses with brain tissue at slaughter. Vet. Rec. 145, 460-462. 
    4. Bozzetta, E., Nappi, R., Ru, G., Negro, M., Maurella, C., and Caramelli, M. (2006) Evaluation of an enzyme immunoassay for the detection of central nervous system tissue contamination at the slaughterhouse. J. Food Prot. 69, 2289-2292. 
    5. Brown, P., Will, R. G., Bradely, R., Asher, D. M., and Detwiler, L. (2001) BSE and variant Creutzfeldt Jakob disease. Br. Med. J. 322,841-844. 
    6. Bruce, M. E., Will, R. G., Ironside, J. W., Mcconnell, I., Drummond, D., Suttie, A., Mccardie, L., Chree, A., Hope, J., Birkett, C., Cousens, S. F. H., and Bostock. C. J. (1997) Transmissions to mice indicate that 'new variant' CJD is caused by the BSE agent. Nature 389, 498-501. 
    7. Choi, S. H., Chae, S. H., Choi, H. H., Kim, J. S., Kang, B. R., Yeo, J. S., Choi, I., Lee, Y. S., Choi, Y. H., and Park, H. S. (2007) Genomic sequence variability of the prion gene (PRNP) in Korean cattle. Asian-Aust. J. Anim. Sci. 20, 653-660. 
    8. Collinge, J., Sidle, K. C. L., Meads, J., Ironside, J., and Hill, A. F. (1996) Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD. Nature 383, 685-690. 
    9. Commission Decision 2000/418/EC.(2000) Regulating the use of material presenting risk as regards transmissible sponge form encephalopathies and amending Decision 94/747/EC. Official J. 158/76. 
    10. Eng, L. F., and Lee, Y. L. (1995) Intermediate filaments in astrocytes. In X. Ketternmann, & X. Ransom, Neuroglia. New York, Oxford University Press. 
    11. EC (European Community) (1998) Relative infectivity of specified risk materials, report of the spongiform encephalopathy assessment committee, Feb, Brussels. 
    12. Hajmeer, M. N., Cliver, O., and Provost, R. (2003) Spinal cord tissue detection in comminuted beef: comparison of two immunological methods. Meat Sci. 65,757-763. 
    13. Hajmeer, M. N., Cliver, O., and Marsden, J. L. (2006) Central nervous system tissue detection in meat from advanced meat recovery systems. Meat Sci. 72, 656-659. 
    14. Hill, A. F., Desbruslais, M., Joiner, S., Sidle, K. C., Gowland, I., Collinge, J., Doey, L. J., and Lantos, P. (1997) The same prion strain causes vCJD and BSE. Nature 389, 448-450. 
    15. Hossner, K. L., Yemm, R. S., Sonnenshein, S. E., Mason, G. L., Cummi, B.A., Reddy, M. C. S., Sofos, J. N., Scanga, J. A., Tatum, J. D., Smith, G. C., and Belk, K. E. (2006) Comparison of immunochemical and immunohistochemical method for the detection of central nervous system tissue in meat products. J. Food Prot. 69, 644-650. 
    16. Hughson, E., Reece, P., Dennis, M, J., and Oehlischlager, S. (2003) Comparative evaluation of the performance of two commercial kits for the detection of CNS tissue in meat. Food Additive Contam. 20, 1034-1043. 
    17. Legname, G., Baskakov, I. V., Cohen, F. E., DeArmond, S. J., and Prusiner, S. B. (2004) Synthetic mammalian prions. Science 305, 673-676. 
    18. Lim, D. G., Erwanto, Y., and Lee, M. (2007a) Comparison of stunning methods in the dissemination of central nervous system tissue on the beef carcass surface. Meat Sci. 75, 622-627. 
    19. Lim, D. G., Kim, D. H., and Lee, M. (2007b) Effect of Spinal Cord Removal before or after Splitting and Washing on CNST Decontamination of Beef Carcasses. Asian-Aust. J. Anim. Sci. 20, 1770-1776. 
    20. Lopes, M., Stopforth, J. D., Sucre, K., Miksch, R. R., Giddens, E., Reddy, M. C. S., Yemm, R. S., and Samadpour, M. (2006) Alternative cutting methods to minimize transfer of nervous system tissue during steak preparation form bonein short loins. J. Food Prot. 69, 1388-1392. 
    21. Lucker, E., Biedermann, W., Lachhab, S. and Heuss, E. (2005) Transmissible spongiform encephalopathy: Assessment of exposure risk in the histological working environment using GC-MS detection of fatty acids as marker for central nervous tissue. Int. J. Hyg. Environ. Health 208, 391-399. 
    22. Scott, M. R., Will, R., Ironside, J., Nguyen, H. O. B., Tremblay, P., DeArmond, S. J., and Prusiner, S. B. (1999) Compelling transgenic evidence for transmission of bovine spongiform encephalopathy prions to humans. Proc. National academy Sci. USA, 96, 15137-15142. 
    23. Schmidt, G. R., Hossner, K. L., and Gould, D. H. (1999) Potential for disruption of central nervous system tissue in beef cattle by different types of captive bolt stunners. J. Food Prot. 62, 390-393. 
    24. Schmidt, G. R., Yemm, R. S., Hossner, K. L., Childs, K. D., O'callaghan, J. P., and Hossner, K. L. (2002) Verification of different glial fibrillary acidic protein analyses as accurate detectors of central nervous system tissue in advanced meat recovery products. Meat Sci. 62, 79-84. 
    25. SSC (The Scientific Steering Committee) (1998) Opinion on the human exposure risk (HER) via food with respect to BSE. Available: http://europa.eu.int/comm/dg24/health/sc/ssc/out67-en.html. 
    26. Verdrager, J. (1996) Creutzfeldt Jakob disease. Lancet 347, 1704. 
    27. Yesilbag, K. and Kalkan, A. (2005) Detection of central nervous system tissues as BSE specified risk material in meat products in Turkey. Food Cont. 16, 11-13. 
    28. WHO (World Health Organization) (1997) Report of a WHO consultation on medicinal and other products in relation to human and animal transmissible spongiform encephalopathies, WHO /EMC/DIS/97.3, Geneva, WHO. 

 저자의 다른 논문

  • Lee, Moo-Ha (57)

    1. 1989 "우육과 돈육의 냉동저장 중 품질변화에 대한 냉동변성 방지제의 첨가효과" 한국식품과학회지 = Korean journal of food science and technology 21 (3): 364~369    
    2. 1990 "해동방법에 따른 해동돈육의 품질변화" 한국식품과학회지 = Korean journal of food science and technology 22 (2): 123~128    
    3. 1990 "압출성형 공정을 이용한 돈피스낵의 제조" 한국식품과학회지 = Korean journal of food science and technology 22 (4): 451~455    
    4. 1990 "압출성형 공정을 이용한 돈육과 계육스낵의 제조" 한국식품과학회지 = Korean journal of food science and technology 22 (4): 456~460    
    5. 1990 "닭고기 model system에서의 인삼의 항산화 효과" 家禽學會誌 = Korean journal of poultry science 17 (2): 123~126    
    6. 1991 "인삼첨가가 돈육과 닭가슴육의 지방산화와 색택에 미치는 영향" 한국식품과학회지 = Korean journal of food science and technology 23 (6): 667~672    
    7. 1992 "돼지고기와 닭고기 지방산화에 대한 인삼의 효과" 한국식품과학회지 = Korean journal of food science and technology 24 (1): 7~10    
    8. 1992 "런천미트 통조림의 품질비교" 한국식품과학회지 = Korean journal of food science and technology 24 (5): 492~496    
    9. 1993 "사후 저장온도 $15^{\circ}C$에서 한우적색근육의 생화학, 물리적 변화에 관한 연구" 한국식품과학회지 = Korean journal of food science and technology 25 (1): 57~62    
    10. 1993 "원료육의 성분 및 기능성 규명을 통한 결착지수의 추정" 한국식품과학회지 = Korean journal of food science and technology 25 (5): 475~480    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • NDSL :

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기