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The journal of microbiology v.50 no.2, 2012년, pp.293 - 300   SCIE SCOPUS 피인용횟수: 1
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Antiviral Activities of Flavonoids Isolated from the Bark of Rhus verniciflua Stokes against Fish Pathogenic Viruses In Vitro

Kang, So-Young    (Department of Aqualife Medicine, Chonnam National University   ); Kang, Ji-Young    (Department of Aqualife Medicine, Chonnam National University   ); Oh, Myung-Joo    (Department of Aqualife Medicine, Chonnam National University  );
  • 초록

    An 80% methanolic extract of Rhus verniciflua Stokes bark showed significant anti-viral activity against fish pathogenic infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) in a cell-based assay measuring virus-induced cytopathic effect (CPE). Activity-guided fractionation and isolation for the 80% methanolic extract of R. verniciflua yielded the most active ethyl acetate fraction, and methyl gallate (1) and four flavonoids: fustin (2), fisetin (3), butin (4) and sulfuretin (5). Among them, fisetin (3) exhibited high antiviral activities against both IHNV and VHSV showing $EC_{50}$ values of 27.1 and $33.3{\mu}M$ with selective indices ( $SI=CC_{50}/EC_{50}$ ) more than 15, respectively. Fustin (2) and sulfuretin (5) displayed significant antiviral activities showing $EC_{50}$ values of $91.2-197.3{\mu}M$ against IHNV and VHSV. In addition, the antiviral activity of fisetin against IHNV and VHSV occurred up to 5 hr post-infection and was not associated with direct virucidal effects in a timed addition study using a plaque reduction assay. These results suggested that the bark of R. verniciflua and isolated flavonoids have significant anti-viral activity against IHNV and VHSV, and also have potential to be used as anti-viral therapeutics against fish viral diseases.


  • 주제어

    Rhus verniciflua .   anti-viral activity .   infectious hematopoietic necrosis virus .   viral hemorrhagic septicemia virus .   flavonoids.  

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  • 이 논문을 인용한 문헌 (1)

    1. Lee, Won-Jae ; Kang, Ji-Eun ; Choi, Ji-Ho ; Jeong, Seok-Tae ; Kim, Myung-Kon ; Choi, Han-Seok 2015. "Comparison of the Flavonoid and Urushiol Content in Different Parts of Rhus verniciflua Stokes Grown in Wonju and Okcheon" 한국식품과학회지 = Korean journal of food science and technology, 47(2): 158~163   

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