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Food science and biotechnology v.21 no.2, 2012년, pp.409 - 417   SCIE SCOPUS
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Carbohydrate Polymers of Chirata (Swertia chirata) Leaves: Structural Features, in vitro Anti-oxidant Activity and Fluorescence Quenching Study

Ghosh, Debjani    (Natural Products Laboratory, Department of Chemistry, The University of Burdwan   ); Bandyopadhyay, Shruti S.    (Natural Products Laboratory, Department of Chemistry, The University of Burdwan   ); Chatterjee, Udipta R.    (Natural Products Laboratory, Department of Chemistry, The University of Burdwan   ); Capek, Peter    (Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences   ); Ray, Bimalendu    (Natural Products Laboratory, Department of Chemistry, The University of Burdwan  );
  • 초록

    Chirata (Swertia chirata) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we analyzed the water-extracted carbohydrate polymer (WECP) of this herb using chemical, chromatographic, and spectroscopic methods. The anti-oxidant capacity of this fraction was studied by ferric reducing anti-oxidant power (FRAP) and DPPH radical assays. Effect of WECP on bovine serum albumin spectrum (BSA) was determined using excitation wavelength of 282 nm. Anion exchange chromatography of WECP yielded 5 fractions (F1-F5) with different chemical compositions. The major fraction (F5) was homogeneous, had an apparent molecular mass of 4.5 kDa, and contains both carbohydrates (57%) and phenolics (34%). The anti-oxidant capacities of WECP and F5 were comparable to standard anti-oxidants. Notably, activities of the carbohydrate polymers (F1-F5) correlate with their phenolics content. Fluorescence quenching measurement suggests that F5 can form complex with BSA and the value of the binding constant is K= $6.28{\times}10^5/M$ .


  • 주제어

    carbohydrate polymer .   structural feature .   anti-oxidant activity .   fluorescence quenching .   chirata (Swertia chirata).  

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