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Interdisciplinary Bio Central v.4 no.2, 2012년, pp.5.1 - 5.9  

Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound

Lee, Jae-Jung    (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research   ); Kim, Jun-Young    (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore   ); Zhai, Duanting    (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore   ); Yun, Seong-Wook    (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research   ); Chang, Young-Tae    (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research  );
  • 초록

    Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.


  • 주제어

    Western blot .   Estern staining .   fluorescence dye BDED .   RC-tag .   peptide chemical tags.  

  • 참고문헌 (17)

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