Lipid composition of symbiosomes from pea root nodules
Abstract The lipid composition of several membrane fractions of pea ( Pisum sativum ) nodule and pea root plasma membrane (RPM) were analysed. The peribacteroid membrane (PBM) contained β-amyrin, a triterpenol that was not accumulated in the RPM. β-Amyrin was also found in the microsomal fraction of nodule cells (MFN), which mostly corresponded to plant cell endoplasmic reticulum vesicles. Δ 5 -Avenasterol, a precursor of other phytosterols, was also accumulated in the PBM as observed in the MFN of pea nodule cells. Treatment of pea plants with a 5-day pulse of 10 μM γ-ketotriazole (an experimental herbicide) resulted in the accumulation of 14α-methyl sterols in the PBM, the MFN and the RPM. The amount of these sterols found in the RPM was ca five times less than in the PBM, suggesting that there was a requirement for large amounts of membrane material from sources other than plasma membrane to synthesize PBM. Phospholipid analysis revealed that both the PBM and the MFN had a greater concentration of phosphatidylcholine (PC) than phosphatidylethanolamine (PE). In the RPM, there was more PE than PC. Analysis of phospholipid-esterified fatty acid indicated that the PBM was more similar to the MFN than to the RPM.
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