Binding and signal transduction of the cloned vascular angiotensin II (AT1a) receptor cDNA stably expressed in Chinese hamster ovary cells
The vascular angiotensin (A) II receptor cDNA (AT 1a ) was transfected into Chinese hamster ovary (CHO) cells to generate the stable cell line CHO-AT 1a . This cell line was used to investigate the binding and signal transduction properties of the cloned vascular AT 1 receptor. Specific binding of sarcosine 1 -[ 125 I]tyrosine 4 -isoleucine 8 -AII ([ 125 I]SI-AII) to CHO-AT 1a membranes reached equilibrium after 1 h at 25 o C and was consistently greater than 95% of total binding. Saturation binding analyses demonstrated [ 125 I]SI-AII bound to a saturable population of sites on membranes with an equilibrium dissociation constant (K D ) of 0.7 nM and a binding site maximum of 1.2 pmol/mg protein. [ 125 I]SI-AII binding to CHO cells was inhibited by the following compounds with a rank order of potency of SI-AII>AII>losartan>AI> >PD 123,177. AII (1 μM) treatment of CHO-AT 1a cells caused an increase in inositol phosphates and intracellular calcium relative to basal levels. These responses were blocked by losartan but not by PD 123,177. AII (1 μM) did not effect adenylate cyclase activity in CHO-AT 1a cells, whereas the agonist inhibited adenylate cyclase activity in rat liver cell membranes. These effects were blocked by 10 μM losartan. These results indicate that CHO-AT 1a cells express functional AT 1a receptors which stimulate phospholipase C activity but not adenylate cyclase activity. CHO-AT 1a cells should provide a useful model for studies of AT 1a receptor domains which are critical to signaling pathways.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기