본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Cell biochemistry and function v.14 no.2, 1996년, pp.149 - 154  

Susceptibility of Gamma‐Irradiated Proteins to In Vitro Glycation: Exposure to Oxygen Free Radicals Increases Glycation‐Induced Modifications

Traverso, Nicola (Institute of General Pathology, University of Genoa, Italy ) ; Odetti, Patrizio (Department of Internal Medicine, University of Genoa, Italy ) ; Cheeseman, Kevin (Department of Biology and Biochemistry, Brunel University, Uxbridge, U.K. ) ; Cottalasso, Damiano (Institute of General Pathology, University of Genoa, Italy ) ; Marinari, Umberto Maria (Institute of General Pathology, University of Genoa, Italy ) ; Pronzato, Maria Adelaide (Institute of General Pathology, University of Genoa, Italy ) ;
  • 초록  

    Abstract Oxidation and glycation are non‐enzymatic protein modifications involved in the pathogenesis of aging. We evaluated their possible influences in an in vitro system: albumin was oxidized by gamma‐irradiation and then exposed to glycation in vitro . Fluorescence modifications were analysed as signals of protein alterations. Both radiolytic oxidation and in vitro glycation provoked a sharp decrease of tryptophan fluorescence (278 nm ex./340 nm em.); their effects tended to be additive, unless a saturation limit was reached. Both individually and in combination, these two non‐enzymatic processes induced the appearance of a new fluorescence (335 nm ex./415 nm em.); in this case as well there was an additive effect, with a trend toward saturation. Radiolytic oxidation and in vitro glycation seem to provoke similar damage to the exposed proteins: the observed fluorescence alterations may be due to similar conformational changes, breaks or the development of fluorophores.


  • 주제어

    glycation .   oxidation .   irradiation .   fluorescence .   albumin .   free radicals.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기