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Antioxidative enzymes activities and lipid peroxidation indicators in blood platelets during bed rest.

Kedziora, J ; Buczyski, A ;
  • 초록  

    Our investigations were carried out on blood platelets obtained from 52 males, aged 25-48, free from any systemic disease, hospitalized because of extremities inferioris fractures without clinical complications. The effect of immobilization on oxygen metabolism and function of human blood platelets was studied. Blood was taken from patients after 14, 28 and 90 days of bed rest and platelets were isolated by a differential centrifugation technique. The comparative control group consisted of 41 healthy individuals at their normal physical activity. Short and long term bed rest had different effect on estimated enzymes activities: SOD-1, GPx-SH, Ct (and lipid peroxidation indicators) (MDA-and TXA2). After 14 and 28 days, the enzyme activities significantly decreased, whereas MDA and TXA2 concentrations increased. It was also found that a 14 and 28-day immobilization increased the blood platelets aggregation. However, after 90 days the tendency towards normalization of all the estimated parameters was observed.


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